Mdm12p, a component required for mitochondrial inheritance that is conserved between budding and fission yeast.
Mdm12p, a component required for mitochondrial inheritance that is conserved between budding and fission yeast.
复制标题
DOI：
10.1083/jcb.136.3.545
复制
发表时间：
1997-02-10
影响因子：
7.8
通讯作者：
Yaffe, MP
中科院分区：
文献类型：
--
作者：
Berger, KH;Sogo, LF;Yaffe, MP
缺乏MDM 12基因产物的酿酒酵母细胞显示温度敏感性生长，并且具有异常大的圆形线粒体，这些线粒体对于子芽的遗传是有缺陷的。对野生型MDM 12基因的分析揭示其产物是与裂殖酵母粟酒裂殖酵母的蛋白质同源的31-kD多肽。当在S. cerevisiae、S.粟酒裂殖酵母Mdm 12 p同源物赋予巨大的线粒体和异常的线粒体分布的显性负表型，表明出芽和裂殖酵母之间Mdm 12 p活性的部分功能保守。色葡萄通过间接免疫荧光显微镜、亚细胞分级分离和免疫检测，将酿酒酵母Mdm 12 p定位于线粒体外膜，并显示完整膜蛋白的生化特性。Mdm 12 p是第三种线粒体外膜蛋白，在S.酿酒酵母和第一个这样的线粒体组成部分，是保守的两个不同的物种。
Saccharomyces cerevisiae cells lacking the MDM12 gene product display temperature-sensitive growth and possess abnormally large, round mitochondria that are defective for inheritance by daughter buds. Analysis of the wild-type MDM12 gene revealed its product to be a 31-kD polypeptide that is homologous to a protein of the fission yeast Schizosaccharomyces pombe. When expressed in S. cerevisiae, the S. pombe Mdm12p homolog conferred a dominant-negative phenotype of giant mitochondria and aberrant mitochondrial distribution, suggesting partial functional conservation of Mdm12p activity between budding and fission yeast. The S. cerevisiae Mdm12p was localized by indirect immunofluorescence microscopy and by subcellular fractionation and immunodetection to the mitochondrial outer membrane and displayed biochemical properties of an integral membrane protein. Mdm12p is the third mitochondrial outer membrane protein required for normal mitochondrial morphology and distribution to be identified in S. cerevisiae and the first such mitochondrial component that is conserved between two different species.
登录
查看更多内容
DOI：
10.1083/jcb.126.6.1375
发表时间：
1994-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Burgess SM;Delannoy M;Jensen RE
通讯作者：
Jensen RE
影响因子：
7.8
作者：
McConnell, S J;Stewart, L C;Talin, A;Yaffe, M P
通讯作者：
Yaffe, M P
影响因子：
3.2
作者：
ITO, H;FUKUDA, Y;KIMURA, A
通讯作者：
KIMURA, A
DOI：
10.1073/pnas.73.4.1083
发表时间：
1976-01-01
影响因子：
11.1
作者：
DOUGLAS, MG;BUTOW, RA
通讯作者：
BUTOW, RA
影响因子：
7.8
作者：
MCCONNELL, SJ;YAFFE, MP
通讯作者：
YAFFE, MP