Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening.
Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening.
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DOI：
10.2174/13862073113166660062
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发表时间：
2014-01
影响因子：
1.8
通讯作者：
Turcatti G
中科院分区：
文献类型：
--
作者：
Rappaz B;Breton B;Shaffer E;Turcatti G
数字全息显微镜（DHM）是一种无标记成像技术，允许用经典的成像细胞培养板观察透明细胞。所提供的定量DHM相衬图像与细胞内折射率和细胞厚度两者相关。 DHM是能够区分细胞形态学变化的两个代表性的细胞系（HeLa和H9c2）时，用阿霉素和氯喹，两种细胞毒性化合物产生不同的表型。我们分析了与细胞形态和终点测量中的细胞内含量相关的参数，并通过时间推移记录对其进行了进一步研究。DHM获得的结果进行了比较与其他光学无标记显微镜技术，即相位对比，微分干涉对比和强度方程的传输（从三个亮场图像重建）。为了比较的目的，在不同的电动显微镜上以常见的96孔板格式获取图像。 与其他显微镜分析相比，使用DHM生成的图像可以使用简单的自动化即时分析方法轻松定量，用于区分每个细胞系中生成的不同表型。DHM技术适用于开发稳健且无偏倚的基于图像的测定。
Digital Holographic Microscopy (DHM) is a label-free imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. The quantitative DHM phase contrast image provided is related both to the intracellular refractive index and to cell thickness. DHM is able to distinguish cellular morphological changes on two representative cell lines (HeLa and H9c2) when treated with doxorubicin and chloroquine, two cytotoxic compounds yielding distinct phenotypes. We analyzed parameters linked to cell morphology and to the intracellular content in endpoint measurements and further investigated them with timelapse recording. The results obtained by DHM were compared with other optical label-free microscopy techniques, namely Phase Contrast, Differential Interference Contrast and Transport of Intensity Equation (reconstructed from three bright-field images). For comparative purposes, images were acquired in a common 96-well plate format on the different motorized microscopes. In contrast to the other microscopies assayed, images generated with DHM can be easily quantified using a simple automatized on-the-fly analysis method for discriminating the different phenotypes generated in each cell line. The DHM technology is suitable for the development of robust and unbiased image-based assays.
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影响因子：
3.7
作者：
Pavillon N;Kühn J;Moratal C;Jourdain P;Depeursinge C;Magistretti PJ;Marquet P
通讯作者：
Marquet P
影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
3.8
作者：
Rappaz, B;Marquet, P;Magistretti, PJ
通讯作者：
Magistretti, PJ
影响因子：
3.5
作者：
Yi, Faliu;Moon, Inkyu;Marquet, Pierre
通讯作者：
Marquet, Pierre
影响因子：
3.5
作者：
Rappaz, Benjamin;Cano, Elena;Marquet, Pierre
通讯作者：
Marquet, Pierre