Early cell death detection with digital holographic microscopy.
Early cell death detection with digital holographic microscopy.
复制标题
DOI：
10.1371/journal.pone.0030912
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Marquet P
中科院分区：
文献类型：
--
作者：
Pavillon N;Kühn J;Moratal C;Jourdain P;Depeursinge C;Magistretti PJ;Marquet P
数字全息术提供了一种非侵入性的测量方法，可以测量细胞在培养过程中引起的定量相移，这可能与细胞体积的变化有关。以前已经表明，细胞体积的调节，特别是与离子稳态有关的调节，在细胞死亡过程的激活/失活中起着至关重要的作用。因此，我们在这里介绍了数字全息显微镜(DHM)的应用，致力于早期和无标记检测细胞死亡。我们提供了由L谷氨酸兴奋性毒性浓度触发的早期神经元体积调节引起的小鼠皮质神经元的相信号的定量测量。我们表明，DHM检测到的这种早期调节细胞体积的效率，与用广泛接受的台盼蓝方法检测细胞活力评估的后续神经元死亡的发生相关。DHM对相位信号的测定为细胞死亡的早期检测提供了一种简单、快速的光学方法。
Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it relates to ionic homeostasis, is crucially involved in the activation/inactivation of the cell death processes. We thus present here an application of digital holographic microscopy (DHM) dedicated to early and label-free detection of cell death. We provide quantitative measurements of phase signal obtained on mouse cortical neurons, and caused by early neuronal cell volume regulation triggered by excitotoxic concentrations of L-glutamate. We show that the efficiency of this early regulation of cell volume detected by DHM, is correlated with the occurrence of subsequent neuronal death assessed with the widely accepted trypan blue method for detection of cell viability. The determination of the phase signal by DHM provides a simple and rapid optical method for the early detection of cell death.
登录
查看更多内容
影响因子：
16.2
作者：
ANKARCRONA, M;DYPBUKT, JM;NICOTERA, P
通讯作者：
NICOTERA, P
影响因子：
5.8
作者：
Duprez, Linde;Wirawan, Ellen;Vandenabeele, Peter
通讯作者：
Vandenabeele, Peter
影响因子：
1.9
作者：
Kemper, Bjoern;von Bally, Gert
通讯作者：
von Bally, Gert
影响因子：
1.9
作者：
Cuche, E;Marquet, P;Depeursinge, C
通讯作者：
Depeursinge, C
影响因子：
2.2
作者：
KORZENIEWSKI, C;CALLEWAERT, DM
通讯作者：
CALLEWAERT, DM