Transcriptome profiling of the spl5 mutant reveals that SPL5 has a negative role in the biosynthesis of serotonin for rice disease resistance.
Transcriptome profiling of the spl5 mutant reveals that SPL5 has a negative role in the biosynthesis of serotonin for rice disease resistance.
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spl5 突变体的转录组分析表明，SPL5 在水稻抗病性血清素的生物合成中具有负面作用
DOI：
10.1186/s12284-015-0052-7
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Ma B
中科院分区：
文献类型：
--
作者：
Jin B;Zhou X;Jiang B;Gu Z;Zhang P;Qian Q;Chen X;Ma B
水稻突变体spl 5（spotted leaf 5）在叶片上具有自发的类过敏性病变，并表现出对病原菌的增强抗性，表明SPL 5在细胞程序性死亡（PCD）和抗病性中起作用。为了了解SPL 5基因的分子机制，我们利用基因芯片技术分别对spl 5突变体叶片（FL）和叶片（ML）与野生型叶片（WT）的转录谱进行了研究。基因芯片结果显示，在spl 5-FL和spl 5-ML中分别有243个和896个候选基因（Fold change ≥ 3.0）表达上调或下调，其中大量基因参与生物防御反应或活性氧代谢。有趣的是，根据我们的芯片和实时PCR检测，转录因子OsWRKY 14和负责5-羟色胺的生物合成的基因，邻氨基苯甲酸合成酶（AS），吲哚-3-甘油磷酸合成酶（IGPS），色氨酸合成酶（TS）和色氨酸脱羧酶（TDC）的表达显着上调spl 5突变体。此前有报道称，水稻中TS和TDC的表达受OsWRKY 14的调控，这增加了OsWRKY 14通过上调TS和TDC来调节5-羟色胺产生的可能性。我们的HPLC分析进一步证实了spl 5突变体叶片中的5-羟色胺水平高于WT。由于5-羟色胺在诱导抗病性中起关键作用，因此增加的5-羟色胺水平可能至少部分地有助于spl 5的抗病性。SPL 5基因可能是激活5-羟色胺代谢途径的负调控因子，这些结果可能为植物中SPL 5诱导的防御反应机制提供新的见解。本文的在线版本（doi：10.1186/s12284-015-0052-7）包含补充材料，可供授权用户使用。
Rice mutant, spl5 (spotted leaf 5), has spontaneous hypersensitive-like lesions on its leaves and shows enhanced resistance to pathogens, indicating that SPL5 plays a role in programmed cell death (PCD) and disease resistance. To understand the molecular mechanism of SPL5 gene, we investigated the transcriptome profiles of the spl5 mutant leaves with few lesions (FL) and leaves with many lesions (ML) compared to the wild-type (WT) leaves respectively by microarray. The data from microarray revealed that 243 and 896 candidate genes (Fold change ≥ 3.0) were up- or down-regulated in the spl5-FL and spl5-ML, respectively, and a large number of these genes involved in biotic defense responses or reactive oxygen species (ROS) metabolism. Interestingly, according to our microarray and real-time PCR assays, the expressions of a transcription factor OsWRKY14 and genes responsible for the biosynthesis of serotonin, anthranilate synthase (AS), indole-3-glycerolphosphate synthase (IGPS), tryptophan synthase (TS) and tryptophan decarboxylase (TDC) were significantly up-regulated in the spl5 mutant. It has been reported previously that TS and TDC expressions are regulated by OsWRKY14 in rice, which raises the possibility that OsWRKY14 regulates serotonin production through the up-regulation of TS and TDC. Our HPLC analysis further confirmed that serotonin levels were higher in the leaves of spl5 mutant than that in WT. Since the serotonin plays a critical role in inducing disease-resistance, the increased serotonin level may contribute, at least partly, to the disease resistance in spl5. The SPL5 gene may act as a negative regulatory factor activating the serotonin metabolic pathway, and these results might provide a new insight into the spl5-induced defense response mechanisms in plants. The online version of this article (doi:10.1186/s12284-015-0052-7) contains supplementary material, which is available to authorized users.
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影响因子：
5.2
作者：
Mizobuchi, R;Hirabayashi, H;Okamoto, M
通讯作者：
Okamoto, M
影响因子：
4.9
作者：
Andi, S;Taguchi, F;Ichinose, Y
通讯作者：
Ichinose, Y
影响因子：
3.1
作者：
Badigannavar, AM;Kale, DM;Murty, GSS
通讯作者：
Murty, GSS
水稻抗病增强病斑模拟突变体斑点叶 5 的蛋白质组学分析
DOI：
10.1186/1939-8433-6-1
发表时间：
2013-01-07
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Chen X;Fu S;Zhang P;Gu Z;Liu J;Qian Q;Ma B
通讯作者：
Ma B
SPL5 是一种细胞死亡和防御相关基因，编码水稻中假定的剪接因子 3b 亚基 3 (SF3b3)
影响因子：
3.1
作者：
Chen, Xifeng;Hao, Liang;Ma, Bojun
通讯作者：
Ma, Bojun