Refining the Galleria mellonella Model by Using Stress Marker Genes to Assess Clostridioides difficile Infection and Recuperation during Phage Therapy.
Refining the Galleria mellonella Model by Using Stress Marker Genes to Assess Clostridioides difficile Infection and Recuperation during Phage Therapy.
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DOI：
10.3390/microorganisms8091306
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发表时间：
2020-08-27
期刊：
影响因子：
4.5
通讯作者：
Clokie MRJ
中科院分区：
文献类型：
--
作者：
Nale JY;Chutia M;Cheng JKJ;Clokie MRJ
Galleria mellonella是探索艰难梭菌与大肠杆菌相互作用的有效模型。尽管从这个模型中获得了有价值的见解，但幼虫并不容易评估对细菌或细菌的详细临床反应。在此，将幼虫存活、定殖和毒素水平与17 G的表达谱进行比较。梅隆菌应激基因监测艰难梭菌感染（CDI），并在噬菌体治疗期间恢复。用核糖体型014/020分离物感染幼虫，并用优化的噬菌体混合物处理。用杀鼠灵处理的幼虫和噬菌体对照组的幼虫都受到保护，表现出最高的存活率和较低的C。difficile殖民和毒素率，相比，共同感染，治疗和细菌控制幼虫组。与共感染、治疗和细菌对照组相比，预防和噬菌体对照幼虫组中生长（9）和繁殖（2）基因的表达增强。相反，感染（2）、体液（1）和细胞（3）免疫基因的表达在预防组和噬菌体对照组中下降，但在共感染、治疗和细菌对照组中增加。分子标记物增加了存活、定殖和毒素数据，并允许详细监测CDI和恢复。这些数据支持使用应激标记基因作为工具来分析该模型中的临床症状。
The Galleria mellonella is an effective model for probing Clostridioides difficile interactions with phages. Despite valuable insights from this model, the larvae are not easily amenable to assessing detailed clinical responses to either bacteria or phages. Here, larval survival, colonisation and toxin levels were compared to expression profiles of 17 G. mellonella stress genes to monitor Clostridiodes difficile infection (CDI), and recuperation during phage therapy. The larvae were infected with a ribotype 014/020 isolate and treated with an optimised phage cocktail. Larvae treated prophylactically with phages and the phage-control larval group were protected, showing the highest survival, and low C. difficile colonisation and toxin rates, compared to co-infection, remedial and bacterial-control larval groups. Expression of growth (9) and reproduction (2) genes were enhanced within prophylaxis and phage-control larval groups compared to the co-infection, remedial and bacterial control groups. In contrast, expression of infection (2), humoral (1) and cellular (3) immunity genes declined in the prophylactic and phage-control groups but increased in the co-infection, remedial and bacterial control larvae. The molecular markers augment the survival, colonisation and toxin data and allow detailed monitoring of CDI and recovery. This data support the use of stress marker genes as tools to analyse clinical symptoms in this model.
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