Post-fasting olfactory, transcriptional, and feeding responses in Drosophila.
Post-fasting olfactory, transcriptional, and feeding responses in Drosophila.
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DOI：
10.1016/j.physbeh.2011.09.007
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发表时间：
2012-01-18
影响因子：
2.9
通讯作者：
Vosshall, Leslie B.
中科院分区：
文献类型：
--
作者：
Farhadian, Shelli F.;Suarez-Farinas, Mayte;Cho, Christine E.;Pellegrino, Maurizio;Vosshall, Leslie B.
禁食一段时间后的饥饿感和进食后的饱腹感对食物摄入的行为调节至关重要，但调节这些感觉的生物学机制尚未完全了解。我们研究了醋蝇（Drosophila melanogaster）对禁食的行为和生理适应。在这里，我们表明，男性和女性的苍蝇增加了他们的食物摄入率短暂的禁食后的状态。虽然基础喂养率是女性高于男性，空腹后喂养反应的幅度是相同的，在两种性别。苍蝇返回到一个稳定的基线摄食率后12小时内返回到食物的男性和24小时的女性。这种喂养的调制伴随着消化系统的作物器官的大小显着增加，这表明禁食的苍蝇通过增加食物摄入量和在作物中储存储备食物来做出反应。苍蝇表现出增加的行为吸引力有吸引力的气味时，食物剥夺。通过微阵列分析的头部、身体和化学感受组织的表达谱显示，禁食24小时后调节415个基因，禁食48小时后调节723个基因，在每个组织和禁食时间点中下调的基因多于上调的基因。这些转录变化表现出丰富的时间动态，并影响了多个功能基因本体论类别的基因。这些观察结果表明，内部生理状态的协调转录反应可以调节摄食行为和食物的化学感觉感知。
The sensation of hunger after a period of fasting and of satiety after eating is crucial to behavioral regulation of food intake, but the biological mechanisms regulating these sensations are incompletely understood. We studied the behavioral and physiological adaptation to fasting in the vinegar fly (Drosophila melanogaster). Here we show that both male and female flies increased their rate of food intake transiently in the post-fasted state. Although the basal feeding rate was higher in females than males, the magnitude of the post-fasting feeding response was the same in both sexes. Flies returned to a stable baseline feeding rate within 12 hr after return to food for males and 24 hr for females. This modulation in feeding was accompanied by a significant increase in the size of the crop organ of the digestive system, suggesting that fasted flies responded both by increasing their food intake and storing reserve food in their crop. Flies demonstrated increased behavioral attraction to an attractive odor when food-deprived. Expression profiling of head, body, and chemosensory tissues by microarray analysis revealed 415 genes regulated by fasting after 24 hr and 723 genes after 48 hr, with downregulated genes outnumbering upregulated genes in each tissue and fasting time point. These transcriptional changes showed rich temporal dynamics and affected genes across multiple functional gene ontology categories. These observations suggest that a coordinated transcriptional response to internal physiological state may regulate both ingestive behaviors and chemosensory perception of food.
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DOI：
10.1073/pnas.0702726104
发表时间：
2007-05-15
影响因子：
11.1
作者：
Ja, William W.;Carvalho, Gil B.;Benzer, Seymour
通讯作者：
Benzer, Seymour
Harshlight：用于微阵列芯片的“矫正化妆”程序。
影响因子：
3
作者：
Suárez-Fariñas, M;Pellegrino, M;Wittkowski, KM;Magnasco, MO
通讯作者：
Magnasco, MO
影响因子：
9.8
作者：
Melcher C;Pankratz MJ
通讯作者：
Pankratz MJ
影响因子：
2.7
作者：
Aime, P.;Duchamp-Viret, P.;Julliard, A. K.
通讯作者：
Julliard, A. K.
影响因子：
5.8
作者：
Wettenhall, JM;Smyth, GK
通讯作者：
Smyth, GK