Effects of Space Flight on Expression of key Proteins in Rice Leaves
Effects of Space Flight on Expression of key Proteins in Rice Leaves
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太空飞行对水稻叶片关键蛋白表达的影响
DOI：
10.1016/j.rsci.2019.12.011
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发表时间：
2020-09
期刊：
影响因子：
4.8
通讯作者：
Sun Yeqing
中科院分区：
文献类型：
--
作者：
Zeng Deyong;Cui Jie;Yin Yishu;Zhang Meng;Shan Shan;Gao Xin;Zhang Yingchun;Lu Weihong;Sun Yeqing
作为一种独特的非生物胁迫形式，外层空间的环境条件预计会引起植物基因组、蛋白质组和代谢途径的变化。然而，外层空间条件在蛋白质一级对植物的总体生理学的影响还有待报告。为探讨太空条件对水稻不同时期生长发育相关生理过程和代谢途径的影响，将水稻品种DN 423的种子搭载SJ-10返回卫星送入轨道12.5d，然后比较处理和对照水稻幼苗的三叶期（TLS）和分蘖期（TS）。除了比较植物生长和活性氧（ROS）水平，幼苗蛋白质组也进行了比较，使用同量异位素标签的相对和绝对定量（iTRAQ）。太空飞行使TLS株高增加了20%，分别降低和增加了TLS和TS幼苗的ROS水平，并分别影响了TLS和TS叶片中36和323蛋白的表达。此外，差异丰度蛋白质的功能主要与代谢，能量和蛋白质的合成和降解。这些结果表明，种子暴露在外层空间条件下会影响随后关键信号蛋白的丰度、基因表达以及蛋白质合成和降解过程，从而影响新陈代谢过程并促进对外层空间非生物压力的适应。因此，本研究揭示了空间飞行对植物的影响，并有助于更全面地了解地外生物学。
As a unique form of abiotic stress, the environmental conditions of outer space are expected to induce changes in plant genomes, proteomes and metabolic pathways. However, the effect of outer space conditions on the overall physiology of plants at the protein level has yet to be reported. To investigate the effects of outer space conditions on the growth- and development-related physiological processes and metabolic pathways of rice different stages, the seeds of rice variety DN423 were sent into orbit for 12.5 d aboard the SJ-10 Returning Satellite, and then the seedlings of both treated and control rice were compared at the three-leaf stage (TLS) and tillering stage (TS). In addition to comparing plant growth and reactive oxygen species (ROS) levels, seedling proteomes were also compared using isobaric tags for relative and absolute quantitation (iTRAQ). Space flight increased TLS plant height by 20%, reduced and increased ROS levels of the TLS and TS seedlings, respectively, and affected the expression of 36 and 323 proteins in TLS and TS leaves, respectively. Furthermore, the functions of the differentially abundant proteins were mainly associated with metabolism, energy, and protein synthesis and degradation. These results suggested that the exposure of seeds to outer space conditions affects the subsequent abundance of key signaling proteins, gene expression, and the processes of protein synthesis and degradation, thereby affecting metabolic processes and promoting adaptation to the abiotic stress of outer space. As such, the present study sheds light on the effects of space flight on plants and contributes to a more comprehensive understanding of extraterrestrial biology.
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DOI：
10.1111/tpj.12473
发表时间：
2014-04
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Lei Zhang;Liqun Du;Liqun Du;Chenjia Shen;Chenjia Shen;Yanjun Yang;Yanjun Yang;B. Poovaiah
通讯作者：
Lei Zhang;Liqun Du;Liqun Du;Chenjia Shen;Chenjia Shen;Yanjun Yang;Yanjun Yang;B. Poovaiah
影响因子：
5.6
作者：
S. Roomi;A. Masi;G. Conselvan;S. Trevisan;S. Quaggiotti;Micaela Pivato;G. Arrigoni;T. Yasmin;P. Carletti
通讯作者：
S. Roomi;A. Masi;G. Conselvan;S. Trevisan;S. Quaggiotti;Micaela Pivato;G. Arrigoni;T. Yasmin;P. Carletti
影响因子：
5.6
作者：
Liu Y;Li J
通讯作者：
Li J
影响因子：
5.6
作者：
Saucedo AL;Hernández-Domínguez EE;de Luna-Valdez LA;Guevara-García AA;Escobedo-Moratilla A;Bojorquéz-Velázquez E;Del Río-Portilla F;Fernández-Velasco DA;Barba de la Rosa AP
通讯作者：
Barba de la Rosa AP
影响因子：
1.6
作者：
M. Durante
通讯作者：
M. Durante