Twenty-six years of chemical fertilization decreased soil RubisCO activity and changed the ecological characteristics of soil cbbL-carrying bacteria in an entisol
Twenty-six years of chemical fertilization decreased soil RubisCO activity and changed the ecological characteristics of soil cbbL-carrying bacteria in an entisol
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26年的化肥降低了土壤RubisCO活性并改变了土壤中携带cbbL的细菌的生态特征
DOI：
10.1016/j.apsoil.2019.05.005
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发表时间：
2019-09
影响因子：
4.8
通讯作者：
Wang Mingxia
中科院分区：
文献类型：
--
作者：
Zhou Zhifeng;Wei Wanling;Shi Xiaojun;Liu Yamin;He Xinhua;Wang Mingxia
土壤自养细菌对于封存大气中的二氧化碳非常重要，因此有助于全球碳循环。然而，关于化学施肥如何改变土壤自养细菌的组成的信息有限。在本研究中，cbbL 基因（卡尔文-本森-巴沙姆循环中的关键基因，编码核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基，RubisCO）用于研究连续受精 26 年的 entisol 中自养细菌的丰度和组成。在此过程中，检查了五种化学施肥方案：无施肥控制（CT）、氮磷施肥（NP）、氮钾施肥（NK）、PK施肥（PK）和氮磷钾施肥（NPK）。结果表明，26年（1991-2017）后，中性pH（7.55）的CT比化肥处理具有更高的RubisCO活性，但cbbL丰度最低。土壤RubisCO活性与土壤pH值呈正相关（R2=0.67，P<0.001）；土壤cbbL丰度与土壤有效氮显着正相关（R2=0.54，P < 0.01），与pH显着负相关（R2=0.30，P < 0.05）。同时，土壤RubisCO活性与cbbL丰度显着负相关（R2=0.67，P < 0.001）。此外，cbbL的α多样性在.CT下最低，表明施肥对土壤cbbL多样性有积极影响。综上所述，26年的长期化学施肥改变了土壤cbbL携带细菌的群落结构，土壤pH和有效氮对土壤cbbL携带细菌的生态特性起着至关重要的控制作用。
Soil autotrophic bacteria are important for sequestrating atmospheric CO2 and thus contribute to the global.carbon cycle. However, information is limited on how chemical fertilization can alter the composition of soil.autotrophic bacteria. In this study, the cbbL gene (a key gene in the Calvin-Benson-Bassham cycle that encodes.the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, RubisCO) was used to study the abundance.and composition of autotrophic bacteria in an entisol with 26 years of continuous fertilization. In this.process, five chemical fertilization regimes were examined: without fertilization control (CT), nitrogen and.phosphorus fertilization (NP), N and potassium fertilization (NK), PK fertilization (PK), and NPK fertilization.(NPK). The results showed that after the 26-year (1991–2017) period, the CT with a neutral pH (7.55) had a.higher RubisCO activity than did the chemical fertilizer treatments, but the cbbL abundance was the lowest.under CT. Soil RubisCO activity positively correlated with soil pH (R2=0.67, P < 0.001); soil cbbL abundance.significantly positively correlated with soil available N (R2=0.54, P < 0.01) and significantly negatively.correlated with pH (R2=0.30, P < 0.05). Meanwhile, the soil RubisCO activity significantly negatively correlated.with cbbL abundance (R2=0.67, P < 0.001). In addition, the alpha-diversity of cbbL was lowest under.CT, indicating a positive effect of fertilization on the soil cbbL diversity. In conclusion, a 26-year long-term.chemical fertilization changed the community structure of soil cbbL-carrying bacteria, and soil pH and available.N played crucial roles in controlling the ecological properties of soil cbbL-carrying bacteria.
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影响因子：
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作者：
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DOI：
--
发表时间：
2000
期刊：
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影响因子：
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