Raman Spectroscopic Imaging of Cholesterol and Docosahexaenoic Acid Distribution in the Retinal Rod Outer Segment.
Raman Spectroscopic Imaging of Cholesterol and Docosahexaenoic Acid Distribution in the Retinal Rod Outer Segment.
复制标题
DOI：
10.1071/ch11019
复制
发表时间：
2011-05-01
影响因子：
1.1
通讯作者：
Schultz ZD
中科院分区：
文献类型：
--
作者：
Schultz ZD
对牛蛙视网膜杆状细胞进行了拉曼振动光谱成像。拉曼光谱可以测定细胞核和细胞内段的富含脂质和蛋白质的棒外段（ROS）。从单个杆状光感受器细胞获得的光谱峰拟合分析显示与胆固醇和含有二十二碳六烯酸的脂类相关的特征振动模式。这些结果提供了杆状光感受器细胞生物分子梯度的直接观察，到目前为止，这是基于间接洗涤剂提取物和组织化学分析的指标，如filipin。检测到的生物分子与整膜蛋白视紫红质的调节有关，能够直接观察这些生物分子的方法为探索它们在细胞过程中的调节作用提供了新的途径。
Raman vibrational spectroscopic imaging was performed on retinal rod cells isolated from bullfrogs (Rana catesbeiana). The Raman spectra enable determination of the lipid and protein rich rod outer segment (ROS) from the nucleus and inner segment of the cell. Peak fitting analysis of spectra obtained from individual rod photoreceptor cells show characteristic vibrational modes that can be associated with cholesterol and docosahexaenoic acid containing lipids. These results provide direct observations of biomolecular gradients in the rod photoreceptor cells, which, thus far, have been based on indirect detergent extracts and histochemical analysis with indicators such as filipin. The detected biomolecules are associated with regulation of the integral membrane protein rhodopsin, and methods capable direct observation of these biomolecules offer new routes to exploring their role in the regulation of cellular processes.
登录
查看更多内容
影响因子：
--
作者：
Krueger, Bettina;Haerteis, Silke;Diakov, Alexei
通讯作者：
Diakov, Alexei
影响因子：
3.4
作者：
Albert, AD;Young, JE;Paw, Z
通讯作者：
Paw, Z
影响因子：
56.9
作者：
Kraft, Mary L.;Weber, Peter K.;Boxer, Steven G.
通讯作者：
Boxer, Steven G.
影响因子：
3.4
作者：
Bennett, Michael P.;Mitchell, Drake C.
通讯作者：
Mitchell, Drake C.
寒冷诱导 T 细胞膜波动处脂筏标记物的微米级和纳米级重组
影响因子：
3.7
作者：
Chen Y;Qin J;Cai J;Chen ZW
通讯作者：
Chen ZW