Collagenase treatment reduces the anisotropy of ultrasonic backscatter in rat myocardium by reducing collagen crosslinks.
Collagenase treatment reduces the anisotropy of ultrasonic backscatter in rat myocardium by reducing collagen crosslinks.
复制标题
DOI：
10.14814/phy2.15849
复制
发表时间：
2023-11
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
--
作者：
心脏中胶原蛋白沉积、降解和交联的失调是由于生理应激增加而发生的。胶原含量与超声反向散射（亮度）相关，我们已经证明反向散射的各向异性可用于测量肌纤维排列，即左心室短轴超声亮度的变化。这项研究调查了胶原蛋白在超声波反向散射各向异性中的作用；雌性 Sprague-Dawley 大鼠心脏用含胶原酶的溶液处理 10 或 30 分钟，或用对照溶液处理 30 分钟。在胶原酶治疗过程中，每隔 2.5 分钟采集一次连续超声图像。从前壁和后壁评估超声反向散射，前壁和后壁的胶原纤维主要垂直于声穿透角排列，并且从侧壁和间隔壁评估，其中胶原蛋白主要平行于声穿透角排列。胶原酶消化减少了心肌内的反向散射各向异性。在整个胶原酶处理过程中，胶原蛋白仍然存在于心肌中，但交联在 10 分钟内发生改变。这些数据表明胶原蛋白的交联调节超声波反向散射的各向异性。各向异性指数源自平行和垂直排列纤维的反向散射差异，可以提供非侵入性指数来监测心肌纤维化的进展和状态。
Dysregulation of collagen deposition, degradation, and crosslinking in the heart occur in response to increased physiological stress. Collagen content has been associated with ultrasonic backscatter (brightness), and we have shown that the anisotropy of backscatter can be used to measure myofiber alignment, that is, variation in the brightness of a left ventricular short‐axis ultrasound. This study investigated collagen's role in anisotropy of ultrasonic backscatter; female Sprague–Dawley rat hearts were treated with a collagenase‐containing solution, for either 10 or 30 min, or control solution for 30 min. Serial ultrasound images were acquired at 2.5‐min intervals throughout collagenase treatment. Ultrasonic backscatter was assessed from anterior and posterior walls, where collagen fibrils are predominately aligned perpendicular to the angle of insonification, and the lateral and septal walls, where collagen is predominately aligned parallel to the angle of insonification. Collagenase digestion reduced backscatter anisotropy within the myocardium. Collagen remains present in the myocardium throughout collagenase treatment, but crosslinking is altered within 10 min. These data suggest that crosslinking of collagen modulates the anisotropy of ultrasonic backscatter. An Anisotropy Index, derived from differences in backscatter from parallel and perpendicularly aligned fibers, may provide a noninvasive index to monitor the progression and state of myocardial fibrosis.
登录
查看更多内容
影响因子：
5.7
作者：
Kwan, Alan;Demosthenes, Emmanuella;Salto, Gerran;Ouyang, David;Nguyen, Trevor;Nwabuo, Chike C.;Luong, Eric;Hoang, Amy;Osypiuk, Ewa;Stantchev, Plamen;Kim, Elizabeth H.;Hiremath, Pranoti;Li, Debiao;Vasan, Ramachandran;Xanthakis, Vanessa;Cheng, Susan
通讯作者：
Cheng, Susan
影响因子：
2.1
作者：
Filova, Elena;Stankova, Lubica;Bacakova, Lucie
通讯作者：
Bacakova, Lucie
影响因子：
2.3
作者：
Milne, Michelle L.;Singh, Gautam K.;Holland, Mark R.
通讯作者：
Holland, Mark R.
影响因子：
2.9
作者：
Milne, Michelle L.;Schick, Brianna M.;Chung, Charles S.
通讯作者：
Chung, Charles S.
影响因子：
37.8
作者：
HOYT, RH;COLLINS, SM;CONYERS, D
通讯作者：
CONYERS, D