Programmable Aggregation of Artificial Cells with DNA Signals
Programmable Aggregation of Artificial Cells with DNA Signals
复制标题
利用 DNA 信号对人工细胞进行可编程聚集
DOI：
10.1021/acssynbio.0c00550
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发表时间：
2021
影响因子：
4.7
通讯作者：
Choi, Jong Hyun
中科院分区：
文献类型：
--
作者：
Qiu, Hengming;Li, Feiran;Du, Yancheng;Li, Ruixin;Hyun, Ji Yeon;Lee, Sei Young;Choi, Jong Hyun
细胞聚集是一种复杂的行为，与细胞的生存能力、分化和迁移密切相关。创造合成类似物的努力可能会导致细胞生理学和生物物理学的重大进步。绘制和调制这样一个动态的人工细胞系统需要接收、转导和传输细胞间信号的机制，但目前有效的工具有限。在这里，我们从工程脂质中构建合成细胞，并使用DNA寡核苷酸作为信号分子显示其可编程聚集行为。人造细胞具有由DNA折纸制成的跨膜通道，用于识别和处理细胞间信号。我们证明，在细胞内酶转导外部DNA信号后，多个小泡聚集在一个大泡上，当接收“释放”信号时，小泡解离。这项工作为构建具有化学通讯和协调能力的合成原细胞提供了新的可能性。
Cell aggregation is a complex behavior that is closely related to the viability, differentiation, and migration of cells. An effort to create synthetic analogs could lead to considerable advances in cell physiology and biophysics. Rendering and modulating such a dynamic artificial cell system require mechanisms for receiving, transducing, and transmitting intercellular signals, yet effective tools are limited at present. Here we construct synthetic cells from engineered lipids and show their programmable aggregation behaviors using DNA oligonucleotides as signaling molecules. The artificial cells have transmembrane channels made of DNA origami that are used to recognize and process intercellular signals. We demonstrate that multiple small vesicles aggregate onto a giant vesicle after a transduction of external DNA signals by an intracellular enzyme and that the small vesicles dissociate when receiving “release” signals. This work provides new possibilities for building synthetic protocells capable of chemical communication and coordination.
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影响因子：
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