Fetal and adult fibroblasts display intrinsic differences in tendon tissue engineering and regeneration.
Fetal and adult fibroblasts display intrinsic differences in tendon tissue engineering and regeneration.
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胎儿和成人成纤维细胞在肌腱组织工程和再生方面表现出内在差异
DOI：
10.1038/srep05515
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发表时间：
2014-07-03
影响因子：
4.6
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Tang QM;Chen JL;Shen WL;Yin Z;Liu HH;Fang Z;Heng BC;Ouyang HW;Chen X
受伤的成人肌腱通过再生过程没有表现出最佳的愈合，而胎儿肌腱可以以再生的方式愈合而不会形成疤痕。因此，我们比较了FF（小鼠胎儿成纤维细胞）和AF（小鼠成人成纤维细胞）作为无支架工程肌腱制造的种子细胞。我们的研究结果表明，FFs有更大的潜力，肌腱组织工程，肌腱相关基因的表达水平较高。在原位损伤模型中，FFs组在愈合后也表现出更好的结构和功能特性，具有更高水平的胶原沉积和更好的微观结构修复。此外，胎儿成纤维细胞可以增加成纤维细胞样细胞的募集，减少炎症细胞在再生过程中向损伤部位的浸润。我们的研究结果表明，更好的再生与FFs的潜在机制，应阐明和用于促进成人肌腱愈合。这可能有助于发展未来的策略来治疗肌腱损伤。
Injured adult tendons do not exhibit optimal healing through a regenerative process, whereas fetal tendons can heal in a regenerative fashion without scar formation. Hence, we compared FFs (mouse fetal fibroblasts) and AFs (mouse adult fibroblasts) as seed cells for the fabrication of scaffold-free engineered tendons. Our results demonstrated that FFs had more potential for tendon tissue engineering, as shown by higher levels of tendon-related gene expression. In thein situAT injury model, the FFs group also demonstrated much better structural and functional properties after healing, with higher levels of collagen deposition and better microstructure repair. Moreover, fetal fibroblasts could increase the recruitment of fibroblast-like cells and reduce the infiltration of inflammatory cells to the injury site during the regeneration process. Our results suggest that the underlying mechanisms of better regeneration with FFs should be elucidated and be used to enhance adult tendon healing. This may assist in the development of future strategies to treat tendon injuries.
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