Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney.
Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney.
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DOI：
10.1016/j.bbrc.2009.06.020
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发表时间：
2009-08-14
影响因子：
3.1
通讯作者：
Zhong, Pei
中科院分区：
文献类型：
--
作者：
Xing, Yifei;Pua, Eric C.;Lu, Xiaochun;Zhong, Pei
关键词：
研究了基于流体力学的基因传递和超声波的协同组合，以实现更好的肾脏基因转移。将编码萤火虫荧光素酶和促红细胞生成素(EPO)基因的真核表达载体单独或联合应用于大鼠左肾，分别应用肾静脉流体动力学注射和超声治疗加或不加超声造影剂。超声波暴露可以提高基于流体动力学的荧光素酶和EPO表达的基因传递效率。当超声强度为2W/cm2、占空比为10%、作用时间为15min时，最大基因表达水平是单独流体动力作用下的4.5倍。基因表达的持续时间、位置和组织特异性不会因超声波暴露而改变。UCA的应用减少了最佳表达所需的超声波处理的强度和暴露时间。适当应用超声和UCA不会改变肾脏的组织结构，也不会损害肾脏的生理功能。
The synergistic combination of hydrodynamics-based gene delivery and ultrasound was investigated to achieve improved gene transfer to the kidney. Plasmids encoding firefly luciferase and Erythropoietin (EPO) gene were delivered into the left kidney of rats by single or combinative application of renal vein hydrodynamic injection and ultrasound treatment with or without the addition of ultrasound contrast agents (UCA). Ultrasound exposure was found to enhance the efficiency of hydrodynamics-based gene delivery for both luciferase and EPO expression. An ultrasound exposure intensity of 2 W/cm2 at 10% duty cycle for 15 min., produced a maximal gene expression 4.5 times higher than hydrodynamic delivery alone. Duration, location, and tissue-specificity of gene expression were not changed by ultrasound exposure. Application of UCA reduced the intensity and exposure duration of ultrasound treatment needed for optimal expression. Appropriate application of ultrasound and UCA did not alter histological structure or impair physiological function of the treated kidney.
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