The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.
The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.
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基于生物信息学分析，DNA拷贝数改变介导的RFC4和GMPS表达上调与ESCC的早期诊断和免疫逃逸相关
DOI：
10.18632/aging.203520
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发表时间：
2021-09-14
期刊：
影响因子：
--
通讯作者：
Huang BJ
中科院分区：
文献类型：
--
作者：
Wang J;Luo FF;Huang TJ;Mei Y;Peng LX;Qian CN;Huang BJ
食管鳞状细胞癌(ESCC)是世界范围内常见的恶性肿瘤。通常情况下，亚洲，特别是中国，食道癌的发病率很高。由于诊断较晚，缺乏有效的治疗方法，食管鳞癌的预后往往较差。为了为ESCC的早期诊断和治疗奠定基础，我们使用了GEO数据库中的基因表达数据集GSE20347和GSE17351以及一个私有数据集来发现ESCC中的差异表达基因(Deg)和关键基因。值得注意的是，我们发现复制因子C亚单位4(RFC4)和鸟氨酸一磷酸合成酶(GMPS)在ESCC中上调，但很少被研究。特别是，据我们所知，我们的研究是第一次探索GMPS和ESCC。此外，我们发现RFC4和GMPS的高水平表达可能是DNA拷贝数改变增加的结果。此外，RFC4和GMPS在食管癌早期和早期淋巴结转移中均呈上调表达。RFC4的表达与GMPS密切相关。此外，我们还探讨了食管癌组织中RFC4和GMPS的表达与肿瘤浸润性免疫细胞(TILs)的关系。结果表明，肿瘤组织中RFC4和GMPS水平升高，部分肿瘤浸润性细胞减少。TIL升高的RFC4和GMPS提示预后较差。综上所述，我们的研究表明，RFC4和GMPS有可能作为ESCC早期诊断的生物标志物，并可能在ESCC的肿瘤免疫过程中发挥关键作用。
Esophageal squamous cell carcinoma (ESCC) is a malignant tumor that commonly occurs worldwide. Usually, Asia, especially China, has a high incidence of esophageal cancer. ESCC often has a poor outcome because of a late diagnosis and lack of effective treatments. To build foundations for the early diagnosis and treatment of ESCC, we used the gene expression datasets GSE20347 and GSE17351 from the GEO database and a private dataset to uncover differentially expressed genes (DEGs) and key genes in ESCC. Notably, we found that replication factor C subunit 4 (RFC4) and guanine monophosphate synthase (GMPS) were upregulated but have been rarely studied in ESCC. In particular, to the best of our knowledge, our study is the first to explore GMPS and ESCC. Furthermore, we found that high levels of RFC4 and GMPS expression may result from an increase in DNA copy number alterations. Furthermore, RFC4 and GMPS were both upregulated in the early stage and early nodal metastases of esophageal carcinoma. The expression of RFC4 was strongly correlated with GMPS. In addition, we explored the relationship between RFC4 and GMPS expression and tumor-infiltrating immune cells (TILs) in esophageal carcinoma. The results showed that the levels of RFC4 and GMPS increased with a decrease in some tumor-infiltrating cells. Upregulated RFC4 and GMPS with high TILs indicate a worse prognosis. In summary, our study shows that RFC4 and GMPS have potential as biomarkers for the early diagnosis of ESCC and may played a crucial role in the process of tumor immunity in ESCC.
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影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
7.7
作者：
Codipilly DC;Qin Y;Dawsey SM;Kisiel J;Topazian M;Ahlquist D;Iyer PG
通讯作者：
Iyer PG
影响因子：
4.7
作者：
Lee, James J.;Natsuizaka, Mitsuteru;Nakagawa, Hiroshi
通讯作者：
Nakagawa, Hiroshi
影响因子：
5.2
作者：
Meng, Ling-Bing;Shan, Meng-Jie;Gong, Tao
通讯作者：
Gong, Tao
影响因子：
12.3
作者：
Li B;Severson E;Pignon JC;Zhao H;Li T;Novak J;Jiang P;Shen H;Aster JC;Rodig S;Signoretti S;Liu JS;Liu XS
通讯作者：
Liu XS