Distinctive left-sided distribution of adrenergic-derived cells in the adult mouse heart.
Distinctive left-sided distribution of adrenergic-derived cells in the adult mouse heart.
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DOI：
10.1371/journal.pone.0022811
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ebert SN
中科院分区：
文献类型：
--
作者：
Osuala K;Telusma K;Khan SM;Wu S;Shah M;Baker C;Alam S;Abukenda I;Fuentes A;Seifein HB;Ebert SN
肾上腺素和去甲肾上腺素在心脏内由神经元和非神经元来源产生。这些肾上腺素能激素对心血管发育和功能有深远的影响，但相对较少的信息是关于肾上腺素能细胞在成人心脏内的具体组织分布。本研究的目的是确定成人心脏内肾上腺素能谱系细胞的解剖定位。为了实现这一点，我们进行了遗传命运作图实验，其中将cre重组酶（Cre）基因插入苯乙醇胺-n-甲基转移酶（Pnmt）基因座的小鼠与纯合Rosa 26报告基因（R26 R）小鼠交叉交配。由于Pnmt作为肾上腺素能细胞的标记基因，这些交配的后代在肾上腺素能谱系细胞中表达β-半乳糖苷酶（βGAL）报告基因。在整个成年小鼠心脏中发现βGAL表达，但主要（89%）位于左心房（LA）和心室（LV）（与RA和RV相比p<0.001），其中许多细胞似乎具有心肌细胞样形态和结构特征。LA的染色模式为弥漫性，但LV游离壁显示出间歇性非随机染色，从心尖延伸至心脏基部，包括沿其周边沿着的前乳头肌重度染色。XGAL+染色的三维计算机辅助重建显示了整个LA和LV的分布，在LV游离壁的中部和顶部区域附近有明显的特异性指状突起。这些数据表明，肾上腺素能衍生的细胞在成年小鼠心脏中显示出独特的左侧分布模式。
Adrenaline and noradrenaline are produced within the heart from neuronal and non-neuronal sources. These adrenergic hormones have profound effects on cardiovascular development and function, yet relatively little information is available about the specific tissue distribution of adrenergic cells within the adult heart. The purpose of the present study was to define the anatomical localization of cells derived from an adrenergic lineage within the adult heart. To accomplish this, we performed genetic fate-mapping experiments where mice with the cre-recombinase (Cre) gene inserted into the phenylethanolamine-n-methyltransferase (Pnmt) locus were cross-mated with homozygous Rosa26 reporter (R26R) mice. Because Pnmt serves as a marker gene for adrenergic cells, offspring from these matings express the β-galactosidase (βGAL) reporter gene in cells of an adrenergic lineage. βGAL expression was found throughout the adult mouse heart, but was predominantly (89%) located in the left atrium (LA) and ventricle (LV) (p<0.001 compared to RA and RV), where many of these cells appeared to have cardiomyocyte-like morphological and structural characteristics. The staining pattern in the LA was diffuse, but the LV free wall displayed intermittent non-random staining that extended from the apex to the base of the heart, including heavy staining of the anterior papillary muscle along its perimeter. Three-dimensional computer-aided reconstruction of XGAL+ staining revealed distribution throughout the LA and LV, with specific finger-like projections apparent near the mid and apical regions of the LV free wall. These data indicate that adrenergic-derived cells display distinctive left-sided distribution patterns in the adult mouse heart.
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影响因子：
5.2
作者：
Ebert SN;Rong Q;Boe S;Pfeifer K
通讯作者：
Pfeifer K
DOI：
10.1007/bf01047451
发表时间：
1990-12-01
期刊：
HISTOCHEMICAL JOURNAL
影响因子：
--
作者：
FORSGREN, S;MORAVEC, M;MORAVEC, J
通讯作者：
MORAVEC, J
影响因子：
--
作者：
Kloberg, AJ;Fritsche, R
通讯作者：
Fritsche, R
影响因子：
15.9
作者：
KENNEDY, B;BIGBY, TD;ZIEGLER, MG
通讯作者：
ZIEGLER, MG
DOI：
10.1002/ar.a.20330
发表时间：
2006-06-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Anderson, Robert H.;Ho, Siew Yen;Lunkenheimer, Paul P.
通讯作者：
Lunkenheimer, Paul P.