Expression systems and species used for transgenic animal bioreactors.
Expression systems and species used for transgenic animal bioreactors.
复制标题
用于转基因动物生物反应器的表达系统和物种
DOI：
10.1155/2013/580463
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发表时间：
2013
影响因子：
--
通讯作者：
Liu E
中科院分区：
文献类型：
--
作者：
Wang Y;Zhao S;Bai L;Fan J;Liu E
转基因动物生物反应器可以生产具有高药用价值的治疗蛋白质。在本文中，我们比较了能够产生治疗性蛋白质的不同系统（细菌、哺乳动物细胞、转基因植物和转基因动物），发现转基因动物是合成药物蛋白质复合物的潜在理想生物反应器。与其他转基因动物表达系统（卵白色、血液、尿液、精浆和蚕茧）相比，转基因动物乳腺具有巨大的潜力。与目前正在研究作为生物反应器的其他哺乳动物物种（猪、山羊、绵羊和牛）相比，兔子具有许多优势：高生育力、容易产生转基因创始人和后代、对朊病毒疾病不敏感、相对较高的产奶量以及不会将严重疾病传播给人类。值得注意的是，对于小型或中型设施，考虑到经济和卫生方面，兔系统是每年生产高达50 kg蛋白质的理想选择;兔是重组蛋白质乳腺特异性表达的有吸引力的候选者。我们还审查了重组蛋白，已产生的靶向表达在兔乳腺和讨论的限制性转基因动物生物反应器。
Transgenic animal bioreactors can produce therapeutic proteins with high value for pharmaceutical use. In this paper, we compared different systems capable of producing therapeutic proteins (bacteria, mammalian cells, transgenic plants, and transgenic animals) and found that transgenic animals were potentially ideal bioreactors for the synthesis of pharmaceutical protein complexes. Compared with other transgenic animal expression systems (egg white, blood, urine, seminal plasma, and silkworm cocoon), the mammary glands of transgenic animals have enormous potential. Compared with other mammalian species (pig, goat, sheep, and cow) that are currently being studied as bioreactors, rabbits offer many advantages: high fertility, easy generation of transgenic founders and offspring, insensitivity to prion diseases, relatively high milk production, and no transmission of severe diseases to humans. Noticeably, for a small- or medium-sized facility, the rabbit system is ideal to produce up to 50 kg of protein per year, considering both economical and hygienic aspects; rabbits are attractive candidates for the mammary-gland-specific expression of recombinant proteins. We also reviewed recombinant proteins that have been produced by targeted expression in the mammary glands of rabbits and discussed the limitations of transgenic animal bioreactors.
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影响因子：
64.8
作者：
HAMMER, RE;PURSEL, VG;BRINSTER, RL
通讯作者：
BRINSTER, RL
影响因子：
46.9
作者：
Dyck, MK;Gagné, D;Pothier, F
通讯作者：
Pothier, F
影响因子：
3.5
作者：
Coulibaly, S;Besenfelder, U;Brem, G
通讯作者：
Brem, G
影响因子：
3
作者：
Aguirre, A;Castro-Palomino, N;Castro, FO
通讯作者：
Castro, FO
影响因子：
3.5
作者：
Bijvoet, AGA;Van Hirtum, H;Reuser, AJJ
通讯作者：
Reuser, AJJ