CCR4-Not Complex Subunit Not2 Plays Critical Roles in Vegetative Growth, Conidiation and Virulence in Watermelon Fusarium Wilt Pathogen Fusarium oxysporum f. sp. niveum.
CCR4-Not Complex Subunit Not2 Plays Critical Roles in Vegetative Growth, Conidiation and Virulence in Watermelon Fusarium Wilt Pathogen Fusarium oxysporum f. sp. niveum.
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DOI：
10.3389/fmicb.2016.01449
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发表时间：
2016
影响因子：
5.2
通讯作者：
Song F
中科院分区：
文献类型：
--
作者：
Dai Y;Cao Z;Huang L;Liu S;Shen Z;Wang Y;Wang H;Zhang H;Li D;Song F
CCR4-NOT复合体是一种多功能调节因子，在真核生物的多种细胞过程中发挥重要作用。本研究对尖孢镰刀菌CCR4-NOT复合体的核心亚单位FonNot2的生物学功能进行了研究。为西瓜枯萎病的病原菌。在接种FON的西瓜根中，FonNot2在分生孢子和萌发分生孢子中的表达水平高于菌丝体。靶向干扰FonNot2基因导致西瓜营养生长受阻，产孢量减少，分生孢子形态异常，致病力降低。侵染行为的扫描电子显微镜观察和植物体内真菌生长的定量逆转录聚合酶链式反应分析表明，ΔFonNot2突变体对西瓜根系的穿透能力存在缺陷，接种植株的根和茎中的真菌生物量减少。表型和生化分析表明，ΔFonNot2突变体在孢子萌发过程中对细胞壁扰动剂(如刚果红和硫代氟白)和氧化胁迫(如H_2O_2和百草枯)表现出超敏反应，降低了镰刀菌酸的含量，并减少了活性氧的产生。我们的研究结果表明，FonNot2通过调控多条途径相关基因的转录调控西瓜细胞壁完整性、氧化胁迫反应、ROS产生和FA生物合成，从而在调控蔬菜生长、分生孢子发生和分生孢子形态以及对西瓜的致病力等方面发挥重要作用。
CCR4-Not complex is a multifunctional regulator that plays important roles in multiple cellular processes in eukaryotes. In the present study, the biological function of FonNot2, a core subunit of the CCR4-Not complex, was explored in Fusarium oxysporum f. sp. niveum (Fon), the causal agent of watermelon wilt disease. FonNot2 was expressed at higher levels in conidia and germinating conidia and during infection in Fon-inoculated watermelon roots than in mycelia. Targeted disruption of FonNot2 resulted in retarded vegetative growth, reduced conidia production, abnormal conidial morphology, and reduced virulence on watermelon. Scanning electron microscopy observation of infection behaviors and qRT-PCR analysis of in planta fungal growth revealed that the ΔFonNot2 mutant was defective in the ability to penetrate watermelon roots and showed reduced fungal biomass in root and stem of the inoculated plants. Phenotypic and biochemical analyses indicated that the ΔFonNot2 mutant displayed hypersensitivity to cell wall perturbing agents (e.g., Congo Red and Calcofluor White) and oxidative stress (e.g., H2O2 and paraquat), decreased fusaric acid content, and reduced reactive oxygen species (ROS) production during spore germination. Our data demonstrate that FonNot2 plays critical roles in regulating vegetable growth, conidiogenesis and conidia morphology, and virulence on watermelon via modulating cell wall integrity, oxidative stress response, ROS production and FA biosynthesis through the regulation of transcription of genes involved in multiple pathways.
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