Gold nanoparticles in injectable calcium phosphate cement enhance osteogenic differentiation of human dental pulp stem cells.
Gold nanoparticles in injectable calcium phosphate cement enhance osteogenic differentiation of human dental pulp stem cells.
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可注射磷酸钙水泥中的金纳米粒子增强人牙髓干细胞的成骨分化
DOI：
10.1016/j.nano.2017.08.014
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Xu HHK
中科院分区：
文献类型：
--
作者：
Xia Y;Chen H;Zhang F;Bao C;Weir MD;Reynolds MA;Ma J;Gu N;Xu HHK
在这项研究中，开发了一种含有金纳米粒子的新型磷酸钙骨水泥（GNP - CPC）。首次研究了它对人牙髓干细胞（hDPSCs）的成骨诱导能力。金纳米粒子的掺入改善了hDPSCs在CPC上的行为，包括更好的细胞黏附（细胞铺展增加约2倍）和增殖，以及增强的成骨分化（在14天时增加约2 - 3倍）。金纳米粒子赋予CPC微纳结构，从而改善细胞黏附和后续行为的表面特性。此外，通过透射电子显微镜（TEM）证实，从GNP - CPC释放的金纳米粒子被hDPSCs内化，从而增强细胞功能。含有金纳米粒子的培养基增强了hDPSCs的细胞活性。这一结果与GNP - CPC的成骨诱导结果一致并提供了支持。总之，GNP - CPC显著增强了hDPSCs的成骨功能。金纳米粒子有望通过纳米形貌修饰CPC，并作为生物活性添加剂从而促进骨再生。
磷酸钙骨水泥（CPC）是一种可注射的支架，在牙科和整形外科手术中具有良好的应用前景。在此，我们通过掺入金纳米粒子（GNPs）溶液对CPC进行了改进。GNP - CPC显著增强了接种的人牙髓干细胞的成骨功能。这些效果归因于GNP - CPC的微纳结构以及细胞对从GNP - CPC释放的金纳米粒子的内化。因此，金纳米粒子在骨组织工程中具有应用前景，并且利用纳米技术修饰CPC可以促进骨再生。
In this study, a novel calcium phosphate cement containing gold nanoparticles (GNP-CPC) was developed. Its osteogenic induction ability on human dental pulp stem cells (hDPSCs) was investigated for the first time. The incorporation of GNPs improved hDPSCs behavior on CPC, including better cell adhesion (about 2-fold increase in cell spreading) and proliferation, and enhanced osteogenic differentiation (about 2–3-fold increase at 14 days). GNPs endow CPC with micro-nano-structure, thus improving surface properties for cell adhesion and subsequent behaviors. In addition, GNPs released from GNP-CPC were internalized by hDPSCs, as verified by transmission electron microscopy (TEM), thus enhancing cell functions. The culture media containing GNPs enhanced the cellular activities of hDPSCs. This result was consistent with and supported the osteogenic induction results of GNP-CPC. In conclusion, GNP-CPC significantly enhanced the osteogenic functions of hDPSCs. GNPs are promising to modify CPC with nanotopography and work as bioactive additives thus enhance bone regeneration. Calcium phosphate cement (CPC) is an injectable scaffold with promising applications in dental and plastic surgeries. Here, we improved CPC by incorporation of a gold nanoparticles (GNPs) solution. GNP-CPC significantly enhanced the osteogenic functions of seeded human dental stem cells. The effects were attributed to the micro-nano-structure of GNP-CPC and the cells’internalization of GNPs released from GNP-CPC. Therefore, GNPs are promising for use in bone tissue engineering, and modifying CPC with nanotechnology can enhance bone regeneration.
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影响因子：
3.9
作者：
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通讯作者：
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影响因子：
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通讯作者：
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DOI：
10.1126/science.1171643
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
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通讯作者：
Zandstra PW