Effects of normobaric oxygen on the progression of focal cerebral ischemia in rats.
Effects of normobaric oxygen on the progression of focal cerebral ischemia in rats.
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DOI：
10.1016/j.expneurol.2013.08.005
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发表时间：
2013-11
影响因子：
5.3
通讯作者：
Lo, Eng H.
中科院分区：
文献类型：
--
作者：
Esposito, Elga;Mandeville, Emiri T.;Hayakawa, Kazuhide;Singhal, Aneesh B.;Lo, Eng H.
常压氧（NBO）减少局灶性脑缺血实验模型中24-48小时的梗死。然而，为了具有临床相关性，必须探索长期安全性和有效性。在此，我们评估了NBO对大鼠短暂局灶性脑缺血后2周胶质细胞活化、神经血管恢复和行为结果的影响。在成年雄性Sprague-Dawley大鼠中，通过大脑中动脉的管腔内闭塞诱导100分钟的短暂局灶性缺血。动物随机分为假手术组、对照组和85′NBO组。对脑容量和行为结果进行盲量化。免疫组化检测NBO对胶质细胞活化和神经血管反应的影响。再灌注2周后，NBO组动物的梗死体积明显缩小。在前肢放置试验、身体摆动试验和减重试验中也有较好的结果。14天后，NBO降低了活化小胶质细胞标志物Iba 1和活化星形胶质细胞标志物GFAP的表达。NBO治疗对血管生成没有可检测的影响。这些结果表明，NBO的保护作用可能持续到中风后2周。
Normobaric oxygen (NBO) reduces infarction at 24–48 hrs in experimental models of focal cerebral ischemia. However, to be clinically relevant, longer term safety and efficacy must be explored. Here, we assessed the effects of NBO on glial activation, neurovascular recovery, and behavioral outcomes at 2 weeks after transient focal ischemia in rats. 100 min transient focal ischemia was induced by intraluminal occlusion of the middle cerebral artery in adult male Sprague-Dawley rats. Animals were randomized into sham, controls or 85′NBO started 15 minutes after ischemic onset. Infarct volumes and behavioral outcomes were blindly quantified. Immunohistochemistry was used to examine the effects of NBO on glial activation and neurovascular responses. After 2 weeks of reperfusion the infarct volume was marked reduced in animals subjected to NBO. They also had better outcomes in forelimb placement test and in body-swing test and weight loss reduction. After 14 days, NBO decreased expression of Iba1, a marker of activated microglia, and GFAP, a marker of activated astrocytes. NBO treatment had no detectable effect on angiogenesis. These results suggest that protective effects of NBO may persist for up to 2 weeks post-stroke.
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