Transcriptional responses of Treponema denticola to other oral bacterial species.
Transcriptional responses of Treponema denticola to other oral bacterial species.
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DOI：
10.1371/journal.pone.0088361
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lux R
中科院分区：
文献类型：
--
作者：
Sarkar J;McHardy IH;Simanian EJ;Shi W;Lux R
Socransky和同事的经典组织将龈下菌斑的口腔细菌分为不同的复合体。齿垢密螺旋体、牙龈卟啉单胞菌和嗜酸坦纳氏菌被归类为与牙周病高度相关的红色复合体。Socransky的工作将红色与橙子复合物种如具核梭杆菌和中间普雷沃氏菌紧密联系在一起，但与其他复合物的成员无关。虽然这些复合物所包含的物种之间的关系部分是由它们彼此物理附着的能力所支持的，但这些相互作用和关联的生理后果尚不清楚。在这项研究中，我们雇用了T.作为一种模式生物，分析接触依赖性反应的相互作用与物种属于同一复杂（牙龈卟啉单胞菌和T。与之密切相关的橙子复合体（使用F. nucleatum和P.intermedia为代表）和不相连的黄色复合物（使用血链球菌和S. gordonii作为代表）。从T.单独的齿垢以及与不同复合物的代表物成对共孵育5小时后，使用微阵列测定各自的基因表达谱。许多与运动、代谢、转运、外膜和假设蛋白相关的基因在T.在测试的伴侣物种的存在下的齿垢。进一步的分析显示，受影响的基因有显著的重叠，我们确定了对其他物种存在的一般反应，这些物种特异于三种复合物中的两种以及单个复合物。最有趣的是，许多预测的主要抗原（如鞭毛，Msp，CTLP）在包括红色复合物的反应中被抑制，表明最密切相关的物种的存在诱导免疫逃避策略。总之，这里提供的数据提供了一个深入的理解，由接触依赖的相互作用，微生物之间的牙周袋栖息的转录反应。
The classic organization by Socransky and coworkers categorized the oral bacteria of the subgingival plaque into different complexes. Treponema denticola, Porphyromonas gingivalis and Tannerella forsythia are grouped into the red complex that is highly correlated with periodontal disease. Socransky's work closely associates red with orange complex species such as Fusobacterium nucleatum and Prevotella intermedia but not with members of the other complexes. While the relationship between species contained by these complexes is in part supported by their ability to physically attach to each other, the physiological consequences of these interactions and associations are less clear. In this study, we employed T. denticola as a model organism to analyze contact-dependent responses to interactions with species belonging to the same complex (P. gingivalis and T. forsythia), the closely associated orange complex (using F. nucleatum and P. intermedia as representatives) and the unconnected yellow complex (using Streptococcus sanguinis and S. gordonii as representatives). RNA was extracted from T. denticola alone as well as after pairwise co-incubation for 5 hrs with representatives of the different complexes, and the respective gene expression profiles were determined using microarrays. Numerous genes related to motility, metabolism, transport, outer membrane and hypothetical proteins were differentially regulated in T. denticola in the presence of the tested partner species. Further analysis revealed a significant overlap in the affected genes and we identified a general response to the presence of other species, those specific to two of the three complexes as well as individual complexes. Most interestingly, many predicted major antigens (e.g. flagella, Msp, CTLP) were suppressed in responses that included red complex species indicating that the presence of the most closely associated species induces immune-evasive strategies. In summary, the data presented here provide an in-depth understanding of the transcriptional responses triggered by contact-dependent interactions between microorganisms inhabiting the periodontal pocket.
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影响因子：
3.1
作者：
OHTA, K;MAKINEN, KK;LOESCHE, WJ
通讯作者：
LOESCHE, WJ
影响因子：
3.1
作者：
KOLENBRANDER, PE;PARRISH, KD;GREENBERG, EP
通讯作者：
GREENBERG, EP
影响因子：
3.2
作者：
Fenno, JC;Wong, GWK;McBride, BC
通讯作者：
McBride, BC
影响因子：
3.2
作者：
Frederick, Jesse R.;Rogers, Elizabeth A.;Marconi, Richard T.
通讯作者：
Marconi, Richard T.
影响因子：
3.8
作者：
Martinez-Pabon, M. C.;Martinez-Gaviria, A.;Tobon-Arroyave, S. I.
通讯作者：
Tobon-Arroyave, S. I.