Chitosan dressing promotes healing in third degree burns in mice: gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling.
Chitosan dressing promotes healing in third degree burns in mice: gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling.
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DOI：
10.1002/jbm.a.34328
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发表时间：
2013-02
影响因子：
4.9
通讯作者：
Baker, Shenda M.
中科院分区：
文献类型：
--
作者：
Baxter, Ruth M.;Dai, Tianhong;Kimball, Jess;Wang, Eugenia;Hamblin, Michael R.;Wiesmann, William P.;McCarthy, Simon J.;Baker, Shenda M.
烧伤是一项重大的健康挑战，愈合后可能会导致疤痕形成。壳聚糖是甲壳素的衍生物，已被用于促进伤口愈合。在这项研究中，我们使用全层皮肤烧伤小鼠模型的基因表达谱来评估使用壳聚糖乳酸盐敷料治疗的益处。受伤三天后，用壳聚糖处理的小鼠表现出与肉芽组织形成相关的基因表达增加。在后来的时间点，即受伤后 7 天，最初表现出表达增加的基因现在下调，并且参与重塑的基因表达增加，表明壳聚糖治疗可加速愈合。定量 RT-PCR 显示壳聚糖敷料调节了 TGFβ1 的 mRNA 水平。 TGFβ1 最初会促进愈合，但长期活动可能会导致疤痕形成。重要的是，我们发现表达在第三天升高，但在第七天降低，表明壳聚糖治疗不会导致疤痕形成，甚至可能有益于预防疤痕形成。此外，TGFβ1表达的双相调节可能成为未来研究基于壳聚糖和其他烧伤伤口治疗方法的伤口愈合潜力的强大生物标志物。 © 2012 Wiley periodicals, Inc. J Biomed Mater Res Part A，2013。
Burns are a significant health challenge and healing can result in scar formation. Chitosan, a derivative of chitin, has been used to promote wound healing. In this study we used gene expression profiling in a mouse model of full thickness cutaneous burn to assess the benefits of treating with a chitosan lactate dressing. Three days after wounding mice treated with chitosan showed increased expression of genes associated with formation of granulation tissue. At a later time point, seven days after wounding, genes that initially showed increased expression were now down‐regulated, and there was increased expression of genes involved in remodeling suggesting that the chitosan treatment results in accelerated healing. Quantitative RT‐PCR showed modulated mRNA levels for TGFβ1 by the chitosan dressing. TGFβ1 initially promotes healing but extended activity can result in scarring. Importantly we found that expression was elevated at day three, but decreased at day seven suggesting that chitosan treatment will not result in scar formation, and may even be beneficial in preventing scar formation. Additionally, the biphasic regulation of expression of TGFβ1 could be a powerful biomarker for future studies of the wound‐healing potential of chitosan based and other treatments for burn wounds. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
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影响因子：
5.7
作者：
Dai T;Tanaka M;Huang YY;Hamblin MR
通讯作者：
Hamblin MR
DOI：
10.1083/jcb.138.1.65
发表时间：
1997-07-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Görlich D;Dabrowski M;Bischoff FR;Kutay U;Bork P;Hartmann E;Prehn S;Izaurralde E
通讯作者：
Izaurralde E
影响因子：
56.9
作者：
Johnson, GL;Lapadat, R
通讯作者：
Lapadat, R
影响因子：
4.6
作者：
MALETTE, WG;QUIGLEY, HJ;RAINER, WG
通讯作者：
RAINER, WG
影响因子：
3.4
作者：
Baldrick, Paul
通讯作者：
Baldrick, Paul