Zinc Oxide Nanoparticle Improves the Intestinal Function of Intrauterine Growth Retardation Finishing Pigs via Regulating Intestinal Morphology, Inflammation, Antioxidant Status and Autophagy.
Zinc Oxide Nanoparticle Improves the Intestinal Function of Intrauterine Growth Retardation Finishing Pigs via Regulating Intestinal Morphology, Inflammation, Antioxidant Status and Autophagy.
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纳米氧化锌通过调节肠道形态、炎症、抗氧化状态和自噬改善宫内生长迟缓肥育猪肠道功能。
DOI：
10.3389/fvets.2022.884945
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发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
本试验旨在研究氧化锌纳米颗粒（Nano-ZnO）对宫内生长迟缓（IUGR）育肥猪生长、免疫、肠道形态和功能的影响。试验获得6头正常出生体重公仔猪（NBW）和12头IUGR公仔猪，21 d断奶。NBW断奶仔猪饲喂基础饲粮（NBW组），IUGR断奶仔猪分为2组，分别饲喂基础饲粮（IUGR组）和在基础饲粮中添加600 mg /kg纳米氧化锌（IUGR+Zn组）。结果表明：(1)与NBW组相比，IUGR组猪在第77天和第163d的体重差异无统计学意义（P < 0.05），但空肠绒毛高度（VH）和绒毛表面积（P < 0.05）显著增加（P < 0.05），白细胞介素-6、TNF-α和NF-κB mRNA表达显著增加（P < 0.05）；与IUGR组相比，饲粮中添加纳米氧化锌对体重无显著影响（P < 0.05），但增加了空肠VH /隐窝深度比和IgA浓度（P < 0.05），降低了空肠TNF-α和NF-κB mRNA表达（P < 0.05）。(2)与NBW组相比，IUGR增加了空肠肿胀的线粒体和自溶酶体数量，增加了固胞体-1 （P62）和微管相关蛋白轻链3B/A （LC3B/A）的蛋白表达（P < 0.05）；与IUGR组相比，纳米氧化锌降低了肿胀的线粒体和自溶酶体的数量，降低了P62和LC3B/A蛋白的表达（P < 0.05）。(3)与NBW组相比，IUGR提高了粘膜丙二醛和蛋白羰基（PC）含量及Keap1蛋白表达量（P < 0.05）；与IUGR组相比，饲粮中添加纳米氧化锌提高了空肠总抗氧化能力、过氧化氢酶、谷胱甘肽过氧化物酶活性和谷胱甘肽含量（P < 0.05），提高了核呼吸因子2 （Nrf2）、谷氨酸-半胱氨酸连接酶修饰子亚基和谷胱甘肽过氧化物酶1 mRNA表达量，提高了总和核Nrf2蛋白表达量（P < 0.05），降低了丙二醛和PC含量以及Keap1蛋白表达量（P < 0.05）。结果表明，IUGR猪在出生后表现出追赶性生长，肠道形态得到改善，饲粮中添加纳米氧化锌可通过空肠Nrf2/Keap1通路进一步改善IUGR猪肠道形态，减少炎症，减少自噬，减轻氧化应激。
This study was to investigate effects of zinc oxide nanoparticle (Nano-ZnO) on growth, immunity, intestinal morphology and function of intrauterine growth retardation (IUGR) finishing pigs. Six normal birth weight (NBW) and 12 IUGR male piglets were obtained and weaned at 21 d. NBW-weaned piglets fed basal diets (NBW group), IUGR-weaned piglets allocated to two groups fed basal diets (IUGR group) and basal diets further supplemented 600 mg Zn/kg from Nano-ZnO (IUGR+Zn group), respectively. All pigs were slaughtered at 163 d. Results showed: (1) IUGR pigs showed no difference in body weight at 77d and 163d (P > 0.05), while had increased villus height (VH) and villus surface area in jejunum (P < 0.05) and enhanced interleukin-6, TNF-α and NF-κB mRNA expression (P < 0.05) as compared to NBW group; Compared with IUGR group, dietary Nano-ZnO did not affect the body weight (P > 0.05), but increased VH to crypt depth ratio and IgA concentration (P < 0.05) and decreased TNF-α and NF-κB mRNA expression in jejunum (P < 0.05). (2) IUGR increased the number of swollen mitochondria and autolysosomes, and protein expressions of sequestosome-1 (P62) and microtubule-associated protein light chain 3 B/A (LC3B/A) in jejunum as compared to NBW group (P < 0.05); Compared with IUGR group, Nano-ZnO decreased the number of swollen mitochondria and autolysosomes, and P62 and LC3B/A protein expression (P < 0.05). (3) IUGR increased mucosal contents of malondialdehyde and protein carbonyl (PC) and Keap1 protein expression (P < 0.05) as compared to NBW group; Compared with IUGR group, dietary Nano-ZnO increased activities of total antioxidant capacity, catalase, glutathione peroxidase, and glutathione content (P < 0.05), and enhanced nuclear respiratory factor 2 (Nrf2), glutamate-cysteine ligase modifier subunit and glutathione peroxidase 1 mRNA expression, and increased total and nuclear Nrf2 protein expression (P < 0.05), and decreased malondialdehyde and PC content, and Keap1 protein expression (P < 0.05) in jejunum. Results suggested that IUGR pigs showed postnatal catch-up growth and improved intestinal morphology, and dietary Nano-ZnO may further improve intestinal morphology, reduce inflammation, decrease autophagy and alleviate oxidative stress via Nrf2/Keap1 pathway in jejunum of IUGR pigs.
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影响因子：
3.3
作者：
Bai, Miaomiao M.;Liu, Hongnan N.;Yin, Yu L.
通讯作者：
Yin, Yu L.
影响因子：
1.8
作者：
Cui, Yiyan;Tian, Zhimei;Ma, Xianyong
通讯作者：
Ma, Xianyong
影响因子：
2.9
作者：
Kazemi, Esmaeil;Nazari, Sajad;Eslamloo, Khalil
通讯作者：
Eslamloo, Khalil
p62/SQSTM1形成自噬降解的蛋白质聚集体，并对亨廷顿蛋白诱导的细胞死亡具有保护作用。
影响因子：
7.8
作者：
Bjorkoy, Geir;Lamark, Trond;Brech, Andreas;Outzen, Heidi;Perander, Maria;Overvatn, Aud;Stenmark, Harald;Johansen, Terje
通讯作者：
Johansen, Terje
NRF2：氧化应激中的INRF2（KEAP1）信号传导。
影响因子：
7.4
作者：
Kaspar, James W.;Niture, Suryakant K.;Jaiswal, Anil K.
通讯作者：
Jaiswal, Anil K.