Synthetic peptides coupled to the surface of liposomes effectively induce SARS coronavirus-specific cytotoxic T lymphocytes and viral clearance in HLA-A*0201 transgenic mice.
Synthetic peptides coupled to the surface of liposomes effectively induce SARS coronavirus-specific cytotoxic T lymphocytes and viral clearance in HLA-A*0201 transgenic mice.
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DOI：
10.1016/j.vaccine.2009.04.001
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发表时间：
2009-06-12
期刊：
影响因子：
5.5
通讯作者：
Matsui M
中科院分区：
文献类型：
--
作者：
Ohno S;Kohyama S;Taneichi M;Moriya O;Hayashi H;Oda H;Mori M;Kobayashi A;Akatsuka T;Uchida T;Matsui M
我们研究了表面连接的脂质体肽是否适用于基于细胞毒性T淋巴细胞（CTL）的疫苗，以对抗严重急性呼吸道综合征（SARS）冠状病毒（SARS-CoV）。我们首先使用HLA-A*0201转基因小鼠和表达预测表位的重组腺病毒鉴定了来自SARS冠状病毒的四个HLA-A *0201限制性CTL表位。将这些肽偶联到脂质体表面，并接种到小鼠体内。其中两个脂质体肽对肽特异性CTL诱导有效，其中一个对表达SARS-CoV表位的痘苗病毒有效，表明表面连接的脂质体肽可能提供有效的基于CTL的SARS疫苗。
We investigated whether the surface-linked liposomal peptide was applicable to a vaccine based on cytotoxic T lymphocytes (CTLs) against severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV). We first identified four HLA-A*0201-restricted CTL epitopes derived from SARS-CoV using HLA-A*0201 transgenic mice and recombinant adenovirus expressing predicted epitopes. These peptides were coupled to the surface of liposomes, and inoculated into mice. Two of the liposomal peptides were effective for peptide-specific CTL induction, and one of them was efficient for the clearance of vaccinia virus expressing epitopes of SARS-CoV, suggesting that the surface-linked liposomal peptide might offer an effective CTL-based vaccine against SARS.
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影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
影响因子：
5.4
作者：
Botten, Jason;Alexander, Jeff;Buchmeier, Michael J.
通讯作者：
Buchmeier, Michael J.
影响因子：
4.7
作者：
Nakano, Y;Mori, M;Uchida, T
通讯作者：
Uchida, T
影响因子：
6.4
作者：
BenMohamed, L;Krishnan, R;Diamond, DJ
通讯作者：
Diamond, DJ