Paxillus involutus-Facilitated Cd(2+) Influx through Plasma Membrane Ca(2+)-Permeable Channels Is Stimulated by H(2)O(2) and H(+)-ATPase in Ectomycorrhizal Populus × canescens under Cadmium Stress.
Paxillus involutus-Facilitated Cd(2+) Influx through Plasma Membrane Ca(2+)-Permeable Channels Is Stimulated by H(2)O(2) and H(+)-ATPase in Ectomycorrhizal Populus × canescens under Cadmium Stress.
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镉胁迫下外生菌根杨中 H2O2 和 H-ATP 酶刺激卷状芽孢杆菌通过质膜 Ca2 渗透通道促进 Cd2 流入
DOI：
10.3389/fpls.2016.01975
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发表时间：
2016
影响因子：
5.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Zhang Y;Sa G;Zhang Y;Zhu Z;Deng S;Sun J;Li N;Li J;Yao J;Zhao N;Zhao R;Ma X;Polle A;Chen S
采用非侵入性微量测试技术，研究了两种卷叶桩菌菌株（MAJ和NAU）无菌培养物中Cd 2+、Ca 2+和H+的通量分布，以及这些真菌与木质Cd 2+超积累植物、Populus形成的外生菌根。canescens和非菌根（NM）根。短期（ST，24 h）或长期（LT，7 d）暴露于50 μM CdCl 2溶液中，经40 min冲击后，真菌菌丝体、NM和外生菌根（EM）根中Cd 2+的内流增加。Cd ~（2+）处理（休克、ST和LT）降低了NM和EM根中的Ca ~（2+）内流，但导致了两个不结球孢菌株无菌生长的EM培养物中Ca ~（2+）内流的增强。真菌菌丝体和白杨根系对Ca 2+通道阻断剂LaCl 3、GdCl 3、异搏定和TEA的敏感性表明，Cd 2+主要通过质膜透性通道进入。Cd ~（2+）处理导致H_2O_2产生。H_2O_2处理促进了Cd ~（2+）和Ca ~（2+）进入NM和EM根系。Cd ~（2+）进一步促进了H ~+泵活性，有利于NM和EM根系维持酸性环境，有利于Cd ~（2+）进入PM。活性氧清除剂DMTU和PM H+-ATP酶抑制剂原钒酸盐（orthovanadate）可降低NM和EM根中Ca ~（2+）和Cd ~（2+）的内流，表明Cd ~（2+）通过Ca ~（2+）渗透通道的进入受到H_2O_2和H ~+泵的刺激。与NM根相比，EM根在冲击、ST和LT Cd ~（2+）处理下表现出更高的Cd ~（2+）通量。结果表明，外生菌根P. × canescens根系在Cd 2+胁迫下保持了显著的H2 O2产生和高的H+泵活性，从而激活了PM Ca 2+通道，促进了Cd 2+的大量内流。
Using a Non-invasive Micro-test Technique, flux profiles of Cd2+, Ca2+, and H+ were investigated in axenically grown cultures of two strains of Paxillus involutus (MAJ and NAU), ectomycorrhizae formed by these fungi with the woody Cd2+-hyperaccumulator, Populus × canescens, and non-mycorrhizal (NM) roots. The influx of Cd2+ increased in fungal mycelia, NM and ectomycorrhizal (EM) roots upon a 40-min shock, after short-term (ST, 24 h), or long-term (LT, 7 days) exposure to a hydroponic environment of 50 μM CdCl2. Cd2+ treatments (shock, ST, and LT) decreased Ca2+ influx in NM and EM roots but led to an enhanced influx of Ca2+ in axenically grown EM cultures of the two P. involutus isolates. The susceptibility of Cd2+ flux to typical Ca2+ channel blockers (LaCl3, GdCl3, verapamil, and TEA) in fungal mycelia and poplar roots indicated that the Cd2+ entry occurred mainly through Ca2+-permeable channels in the plasma membrane (PM). Cd2+ treatment resulted in H2O2 production. H2O2 exposure accelerated the entry of Cd2+ and Ca2+ in NM and EM roots. Cd2+ further stimulated H+ pumping activity benefiting NM and EM roots to maintain an acidic environment, which favored the entry of Cd2+ across the PM. A scavenger of reactive oxygen species, DMTU, and an inhibitor of PM H+-ATPase, orthovanadate, decreased Ca2+ and Cd2+ influx in NM and EM roots, suggesting that the entry of Cd2+ through Ca2+-permeable channels is stimulated by H2O2 and H+ pumps. Compared to NM roots, EM roots exhibited higher Cd2+-fluxes under shock, ST, and LT Cd2+ treatments. We conclude that ectomycorrhizal P. × canescens roots retained a pronounced H2O2 production and a high H+-pumping activity, which activated PM Ca2+ channels and thus facilitated a high influx of Cd2+ under Cd2+ stress.
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丛枝菌根真菌对青杨铅污染性别特异性反应的影响
影响因子：
6.8
作者：
Chen, Lianghua;Hu, Xiangwei;Gao, Shun
通讯作者：
Gao, Shun
影响因子：
2.6
作者：
Danielsen, L.;Thuermer, A.;Meinicke, P.;Buee, M.;Morin, E.;Martin, F.;Pilate, G.;Daniel, R.;Polle, A.;Reich, M.
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Reich, M.
影响因子：
3.7
作者：
Danielsen L;Lohaus G;Sirrenberg A;Karlovsky P;Bastien C;Pilate G;Polle A
通讯作者：
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影响因子：
7.4
作者：
Grabov, A;Blatt, MR
通讯作者：
Blatt, MR
影响因子：
4.4
作者：
Courbot, M;Diez, L;Leroy, P
通讯作者：
Leroy, P