Ectomycorrhizal colonization and diversity in relation to tree biomass and nutrition in a plantation of transgenic poplars with modified lignin biosynthesis.
Ectomycorrhizal colonization and diversity in relation to tree biomass and nutrition in a plantation of transgenic poplars with modified lignin biosynthesis.
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DOI：
10.1371/journal.pone.0059207
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Polle A
中科院分区：
文献类型：
--
作者：
Danielsen L;Lohaus G;Sirrenberg A;Karlovsky P;Bastien C;Pilate G;Polle A
来自杨树等快速生长树种的生物质种植园的木材可用作生产生物燃料的替代原料。为了促进利用木质纤维素进行糖化，具有改变的或降低的木质素含量的转基因杨树可能是有用的。然而，潜在的影响，杨树改造的木质化途径外生菌根（EM）真菌，发挥重要作用的植物营养，是未知的。本研究的目的是调查EM殖民和社区组成的关系，生物量和营养状况的野生型（WT，山杨×白杨）和转基因杨树线的抑制活性的肉桂醇脱氢酶，咖啡酸/5-羟基阿魏酸O-甲基转移酶，肉桂酰CoA还原酶在生物量种植园。在不同的一年生白杨品系中，EM定殖率从58%到86%不等，但WT和转基因杨树的EM群落组成没有区别。两年后，所有品系的定殖率增加到约100%，但观察到不同转基因白杨基因型之间的EM群落分离。EM组合的分化与欧美杨不同基因型无性系间的分化相似。转基因杨树在木材生长和营养元素方面表现出显着差异，生物量较低的基因型茎中养分积累量普遍高于生物量较高的基因型。一个一般的线性混合模型模拟一年生白杨茎的生物量与高精度（调整R2 = 97%）的两个因素：EM殖民和逆木材N浓度。 这些结果意味着N分配和EM定殖之间的联系，这可能是至关重要的木材生产在建立阶段的白杨生物质人工林。我们的数据进一步支持，多个白杨基因型，无论是通过转基因方法或常规育种产生的生物质人工林EM群落组成的变化增加。
Wood from biomass plantations with fast growing tree species such as poplars can be used as an alternative feedstock for production of biofuels. To facilitate utilization of lignocellulose for saccharification, transgenic poplars with modified or reduced lignin contents may be useful. However, the potential impact of poplars modified in the lignification pathway on ectomycorrhizal (EM) fungi, which play important roles for plant nutrition, is not known. The goal of this study was to investigate EM colonization and community composition in relation to biomass and nutrient status in wildtype (WT, Populus tremula × Populus alba) and transgenic poplar lines with suppressed activities of cinnamyl alcohol dehydrogenase, caffeate/5-hydroxyferulate O-methyltransferase, and cinnamoyl-CoA reductase in a biomass plantation. In different one-year-old poplar lines EM colonization varied from 58% to 86%, but the EM community composition of WT and transgenic poplars were indistinguishable. After two years, the colonization rate of all lines was increased to about 100%, but separation of EM communities between distinct transgenic poplar genotypes was observed. The differentiation of the EM assemblages was similar to that found between different genotypes of commercial clones of Populus × euramericana. The transgenic poplars exhibited significant growth and nutrient element differences in wood, with generally higher nutrient accumulation in stems of genotypes with lower than in those with higher biomass. A general linear mixed model simulated biomass of one-year-old poplar stems with high accuracy (adjusted R2 = 97%) by two factors: EM colonization and inverse wood N concentration. These results imply a link between N allocation and EM colonization, which may be crucial for wood production in the establishment phase of poplar biomass plantations. Our data further support that multiple poplar genotypes regardless whether generated by transgenic approaches or conventional breeding increase the variation in EM community composition in biomass plantations.
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影响因子：
3.7
作者：
Baum, C;Stetter, U;Makeschin, F
通讯作者：
Makeschin, F
影响因子：
4.9
作者：
Bradley, Kate L.;Hancock, Jessica E.;Pregitzer, Kurt S.
通讯作者：
Pregitzer, Kurt S.
影响因子：
1.9
作者：
Glynn, C;Rönnberg-Wästljung, AC;Weih, M
通讯作者：
Weih, M
影响因子：
1.6
作者：
Holeski, Liza M.;Vogelzang, Andrew;Lindroth, Richard L.
通讯作者：
Lindroth, Richard L.
影响因子：
2.6
作者：
Danielsen, L.;Thuermer, A.;Meinicke, P.;Buee, M.;Morin, E.;Martin, F.;Pilate, G.;Daniel, R.;Polle, A.;Reich, M.
通讯作者：
Reich, M.