Generation of tissue constructs for cardiovascular regenerative medicine: from cell procurement to scaffold design.
Generation of tissue constructs for cardiovascular regenerative medicine: from cell procurement to scaffold design.
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DOI：
10.1016/j.biotechadv.2012.08.006
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发表时间：
2013-09
影响因子：
16
通讯作者：
Murthy, Shashi K.
中科院分区：
文献类型：
--
作者：
Tandon, Vishal;Zhang, Boyang;Radisic, Milica;Murthy, Shashi K.
关键词：
人体从冠心病中自然恢复的能力是有限的，因为心脏细胞是终末分化的，具有低增殖率和低周转率。心血管组织工程提供了离体生产心脏组织的潜力，但目前受到几个挑战的限制：（i）组织工程构建体需要纯的种子细胞群体，（ii）具有与天然组织中存在的相同长度尺度的特征的3-D几何结构的制造是不平凡的，以及（iii）细胞需要来自适当的生物、电学和机械因素的刺激。在这篇综述中，我们总结了当前状态的微流控技术富集所需的心血管组织工程细胞亚群，提供了独特的优势，传统的平板和基于FACS/MACS的富集。然后，我们总结了现代技术生产组织工程支架，模仿天然心脏组织。
The ability of the human body to naturally recover from coronary heart disease is limited because cardiac cells are terminally differentiated, have low proliferation rates, and low turnover rates. Cardiovascular tissue engineering offers the potential for production of cardiac tissue ex vivo but is currently limited by several challenges: (i) Tissue engineering constructs require pure populations of seed cells, (ii) Fabrication of 3-D geometrical structures with features of the same length scales that exist in native tissue is non-trivial, and (iii) Cells require stimulation from the appropriate biological, electrical and mechanical factors. In this review, we summarize the current state of microfluidic techniques for enrichment of subpopulations of cells required for cardiovascular tissue engineering, which offer unique advantages over traditional plating and FACS/MACS-based enrichment. We then summarize modern techniques for producing tissue engineering scaffolds that mimic native cardiac tissue.
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DOI：
10.1021/la105016a
发表时间：
2011-04-05
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Hatch A;Hansmann G;Murthy SK
通讯作者：
Murthy SK
影响因子：
6.1
作者：
Au, Hoi Ting Heidi;Cui, Bo;Radisic, Milica
通讯作者：
Radisic, Milica
影响因子：
4.8
作者：
Eschenhagen, T;Fink, C;Elson, EL
通讯作者：
Elson, EL
影响因子：
17.1
作者：
Damani S;Bacconi A;Libiger O;Chourasia AH;Serry R;Gollapudi R;Goldberg R;Rapeport K;Haaser S;Topol S;Knowlton S;Bethel K;Kuhn P;Wood M;Carragher B;Schork NJ;Jiang J;Rao C;Connelly M;Fowler VM;Topol EJ
通讯作者：
Topol EJ
影响因子：
4.1
作者：
Boudou, Thomas;Legant, Wesley R.;Chen, Christopher S.
通讯作者：
Chen, Christopher S.