Gold Nanomaterial System That Enables Dual Photothermal and Chemotherapy for Breast Cancer.
Gold Nanomaterial System That Enables Dual Photothermal and Chemotherapy for Breast Cancer.
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DOI：
10.3390/pharmaceutics15092198
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发表时间：
2023-08-25
期刊：
影响因子：
5.4
通讯作者：
Tang L
中科院分区：
文献类型：
--
作者：
Wang L;Shrestha B;Brey EM;Tang L
本研究涉及多功能治疗纳米复合材料系统的制造和表征，以及其在乳腺癌治疗的体外疗效评估。纳米复合材料系统结合了金纳米棒（GNRs）和金纳米团簇（GNC），以实现光热疗法和基于阿霉素的化疗的组合。合成了各种尺寸但表现出相似吸收光谱的GNRs，并筛选了光热效率。选择表现出最高光热效率的GNR用于进一步的实验。在牛血清白蛋白（BSA）中合成GNC，并使用逐层组装将其整合到柠檬酸盐封端的GNR中。采用戊二醛与BSA中的赖氨酸残基交联，将GNC固定在GNR上，形成稳定的“软凝胶状”结构。这种结构通过静电相互作用为阿霉素提供了结合位点，并增强了纳米复合材料的整体结构稳定性。此外，GNC的存在允许纳米复合物系统在~520 nm至700 nm范围内发射稳健的荧光以用于自检测。在纳米复合物的外表面上将透明质酸官能化作为CD 44的靶向部分，以改善乳腺癌细胞的细胞内化和特异性。所开发的纳米复合材料系统在体外表现出良好的稳定性，并表现出pH和近红外响应的药物释放行为。体外研究表明，在近红外辐射后，MDA-MB-231乳腺癌细胞中纳米复合物系统的有效内化和细胞活力降低。总之，这些结果突出了这种纳米复合材料系统用于靶向乳腺癌治疗的潜力。
This study involves the fabrication and characterization of a multifunctional therapeutic nanocomposite system, as well as an assessment of its in vitro efficacy for breast cancer treatment. The nanocomposite system combines gold nanorods (GNRs) and gold nanoclusters (GNCs) to enable a combination of photothermal therapy and doxorubicin-based chemotherapy. GNRs of various sizes but exhibiting similar absorbance spectra were synthesized and screened for photothermal efficiency. GNRs exhibiting the highest photothermal efficiency were selected for further experiments. GNCs were synthesized in bovine serum albumin (BSA) and integrated into citrate-capped GNRs using layer-by-layer assembly. Glutaraldehyde crosslinking with the lysine residues in BSA was employed to immobilize the GNCs onto the GNRs, forming a stable “soft gel-like” structure. This structure provided binding sites for doxorubicin through electrostatic interactions and enhanced the overall structural stability of the nanocomposite. Additionally, the presence of GNCs allowed the nanocomposite system to emit robust fluorescence in the range of ~520 nm to 700 nm for self-detection. Hyaluronic acid was functionalized on the exterior surface of the nanocomposite as a targeting moiety for CD44 to improve the cellular internalization and specificity for breast cancer cells. The developed nanocomposite system demonstrated good stability in vitro and exhibited a pH- and near-infrared-responsive drug release behavior. In vitro studies showed the efficient internalization of the nanocomposite system and reduced cellular viability following NIR irradiation in MDA-MB-231 breast cancer cells. Together, these results highlight the potential of this nanocomposite system for targeted breast cancer therapy.
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影响因子：
3.9
作者：
Liu, Kang;Zheng, Yuanhui;Gooding, J. Justin
通讯作者：
Gooding, J. Justin
DOI：
10.1073/pnas.1703151114
发表时间：
2017-07-11
影响因子：
11.1
作者：
Ali, Moustafa R. K.;Wu, Yue;El-Sayed, Mostafa A.
通讯作者：
El-Sayed, Mostafa A.
影响因子：
38.3
作者：
Loynachan, Colleen N.;Soleimany, Ava P.;Stevens, Molly M.
通讯作者：
Stevens, Molly M.
用于荧光成像引导的光动力疗法的光敏剂共轭二氧化硅涂层金纳米团簇。
影响因子：
14
作者：
Huang, Peng;Lin, Jing;Wang, Shouju;Zhou, Zhijun;Li, Zhiming;Wang, Zhe;Zhang, Chunlei;Yue, Xuyi;Niu, Gang;Yang, Min;Cui, Daxiang;Chen, Xiaoyuan
通讯作者：
Chen, Xiaoyuan
影响因子：
15.6
作者：
Guzman, Eduardo;Mateos-Maroto, Ana;Rubio, Ramon G.
通讯作者：
Rubio, Ramon G.