Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei.
Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei.
复制标题
乙酸盐转运蛋白的实验证据以及马氏甲烷八叠球菌乙酸盐产甲烷过程中乙酸盐活化的表征
DOI：
10.1111/1574-6968.12550
复制
发表时间：
2014
影响因子：
2.1
通讯作者：
Deppenmeier U
中科院分区：
文献类型：
--
作者：
Welte C;Kröninger L;Deppenmeier U
产乙酸甲烷菌将乙酸代谢为甲烷和二氧化碳。这些生物体的中心代谢和电子传递链已经被研究。然而，没有特别注意到醋酸盐进入古细菌细胞的机制。本研究对马氏甲烷八叠球菌乙酸激酶（Ack）和乙酸摄取反应进行了研究。在2 mM醋酸盐浓度下，M. Mazei不限制甲烷形成速率。相反，产甲烷速率由底物浓度控制，并且在10 mM乙酸盐下增加10倍。随后，我们使用相应的缺失突变体分析了推定的乙酸通透酶MM_0903的参与。在2mM乙酸盐下，在突变体中仅测量到野生型甲烷形成速率的25%。这表明向Ack供应乙酸盐限制了甲烷形成的速率。此外，突变体显示了增加的乙酸激酶活性与野生型相比。这些结果首次表明醋酸盐转运蛋白参与醋酸破细胞产甲烷，并且可能是甲烷肉瘤产甲烷醋酸盐阈值浓度的重要因素。
Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigatedMethanosarcina mazeiacetate kinase (Ack) and the acetate uptake reaction. At a concentration of 2 mM acetate, the Ack activity in cell extract ofM. mazeiwas not limiting for the methane formation rate. Instead, the methanogenesis rate was controlled by the substrate concentration and increased 10-fold at 10 mM acetate. Subsequently, we analyzed the involvement of the putative acetate permease MM_0903 using a corresponding deletion mutant. At 2 mM acetate, only 25% of the wild-type methane formation rate was measured in the mutant. This indicated that the supply of acetate to Ack was limiting the rate of methane formation. Moreover, the mutant revealed an increased acetate kinase activity compared with the wild type. These results show for the first time that an acetate transporter is involved in aceticlastic methanogenesis and may be an important factor in the acetate threshold concentration for methanogenesis ofMethanosarcinaspp.
登录
查看更多内容
影响因子：
4.2
作者：
Rohlin L;Gunsalus RP
通讯作者：
Gunsalus RP
DOI：
10.1016/j.bbabio.2013.12.002
发表时间：
2014-07
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
C. Welte;U. Deppenmeier
通讯作者：
C. Welte;U. Deppenmeier
乙醇和其他烷醇对酿酒酵母中乙酸转运的影响
影响因子：
4.4
作者：
M. Casal;H. Cardoso;C. Leão
通讯作者：
C. Leão
影响因子：
3.1
作者：
Hovey, R;Lentes, S;Deppenmeier, U
通讯作者：
Deppenmeier, U
嗜热甲烷八叠球菌乙酸激酶活性位点残基的定点突变分析*
影响因子：
4.8
作者：
R. D. Miles;P. Iyer;J. Ferry
通讯作者：
J. Ferry