Rice slender leaf 1 gene encodes cellulose synthase-like D4 and is specifically expressed in M-phase cells to regulate cell proliferation.
Rice slender leaf 1 gene encodes cellulose synthase-like D4 and is specifically expressed in M-phase cells to regulate cell proliferation.
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DOI：
10.1093/jxb/ert060
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发表时间：
2013-04
影响因子：
6.9
通讯作者：
Nagato Y
中科院分区：
文献类型：
--
作者：
Yoshikawa T;Eiguchi M;Hibara K;Ito J;Nagato Y
纤维素脱羧酶样（CSL）基因被预测催化非纤维素多糖如半纤维素的β-d-聚糖骨架的生物合成，并且被分类为9个亚家族（CSLA-CSLH和CSLJ）。CSLD亚家族在所有陆生植物中是保守的，在9个CSL亚家族中，它与纤维素合成酶基因的序列相似性最高，表明它在植物发育中起着重要作用。本研究对水稻细长叶1（sle 1）突变体进行了详细的分析，这些突变体表现出卷曲和窄叶片以及株高降低。sle 1的窄叶片是由P3原始阶段开始的细胞增殖减少引起的。除了器官体积缩小外，sle 1突变体在花粉形成、花药开裂、气孔形成和各种组织细胞排列方面表现出严重的发育缺陷。图位克隆结果表明，SLE 1编码OsCSLD 4蛋白，该蛋白是从一个窄叶矮秆突变体中鉴定的。原位杂交结果表明，OsCSLD 4基因在发育器官中呈斑片状表达。双靶原位杂交和定量RT-PCR分析表明，SLE 1的表达特异性在M期的细胞周期，并建议改变sLE 1突变体的细胞周期调控。这些结果表明，OsCSLD 4蛋白在M期发挥关键作用，以调节细胞增殖。对OsCSLD 4的进一步研究有望对半纤维素在植物发育中的作用产生新的认识。
Cellulose synthase-like (CSL) genes are predicted to catalyse the biosynthesis of non-cellulosic polysaccharides such as the β-d-glycan backbone of hemicelluloses and are classified into nine subfamilies (CSLA–CSLH and CSLJ). The CSLD subfamily is conserved in all land plants, and among the nine CSL subfamilies, it shows the highest sequence similarity to the cellulose synthase genes, suggesting that it plays fundamental roles in plant development. This study presents a detailed analysis of slender leaf 1 (sle1) mutants of rice that showed rolled and narrow leaf blades and a reduction in plant height. The narrow leaf blade of sle1 was caused by reduced cell proliferation beginning at the P3 primordial stage. In addition to the size reduction of organs, sle1 mutants exhibited serious developmental defects in pollen formation, anther dehiscence, stomata formation, and cell arrangement in various tissues. Map-based cloning revealed that SLE1 encodes the OsCSLD4 protein, which was identified previously from a narrow leaf and dwarf 1 mutant. In situ hybridization experiments showed that OsCSLD4 was expressed in a patchy pattern in developing organs. Double-target in situ hybridization and quantitative RT-PCR analyses revealed that SLE1 was expressed specifically during the M-phase of the cell cycle, and suggested that the cell-cycle regulation was altered in sle1 mutants. These results suggest that the OsCSLD4 protein plays a pivotal role in the M phase to regulate cell proliferation. Further study of OsCSLD4 is expected to yield new insight into the role of hemicelluloses in plant development.
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影响因子：
7.2
作者：
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通讯作者：
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