Subinhibitory Antibiotic Concentrations Enhance Biofilm Formation of Clinical Enterococcus faecalis Isolates.
Subinhibitory Antibiotic Concentrations Enhance Biofilm Formation of Clinical Enterococcus faecalis Isolates.
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DOI：
10.3390/antibiotics10070874
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发表时间：
2021-07-19
期刊：
影响因子：
--
通讯作者：
Al-Ahmad A
中科院分区：
文献类型：
--
作者：
Bernardi S;Anderson A;Macchiarelli G;Hellwig E;Cieplik F;Vach K;Al-Ahmad A
粪肠球菌是一种可在口腔中发现的微生物，尤其是在继发性牙髓感染中，患病率为24- 70%。在亚抑制抗生素浓度存在下形成生物膜的能力增加是一种在多种细菌病原体中观察到的现象，并且与增加的抗性相关。因此，在本研究中，6 E.将来自牙髓环境的粪链球菌分离株和两种对照菌株暴露于亚抑制浓度的青霉素G、阿莫西林、强力霉素、磷霉素、四环素和万古霉素，并检查它们的生物膜形成能力。测定各菌株的最低抑菌浓度（MIC）。粪杆菌分离株。将分离物的培养物与相应抗生素的连续稀释系列混合，孵育过夜，并使用微量滴定板测定法分析生物膜形成。所有分离株在不存在抗生素的情况下都能够形成生物膜。在暴露于各种抗生素的亚抑制浓度的分离株中发现生物膜形成的显着增加高达50%以上。大多数菌株显示磷霉素（7/8）、强力霉素（6/8）和四环素（6/8）显著增加。三个牙髓分离株显示出显着增加，在同一时间检查的抗生素。暴露于万古霉素，三个牙髓分离株和两个对照菌株显示增加。形成生物膜的能力的增加在MIC浓度的1/2至1/64的浓度范围内延伸。抗生素只有在亚抑菌浓度下才能到达口腔中的某些小生境。这可以增加肠球菌的生物膜形成，进而导致这些分类群对抗生素的敏感性降低。
Enterococcus faecalis is a microorganism that can be found in the oral cavity, especially in secondary endodontic infections, with a prevalence ranging from 24–70%. The increase in the ability to form biofilms in the presence of subinhibitory antibiotic concentrations is a phenomenon that is observed for a wide variety of bacterial pathogens and is associated with increased resistance. In this study, therefore, six E. faecalis isolates from an endodontic environment and two control strains were exposed to subinhibitory concentrations of Penicillin G, Amoxicillin, Doxycycline, Fosfomycin, Tetracycline and Vancomycin and examined for their biofilm formation abilities. The minimum inhibitory concentration (MIC) was determined for all E. faecalis isolates. A culture of the isolate was mixed with a serial dilution series of the respective antibiotic, incubated overnight and the biofilm formation was analyzed using a microtiter plate assay. All isolates were able to form biofilms in the absence of an antibiotic. A significant increase in biofilm formation of up to more than 50% was found in the isolates exposed to subinhibitory concentrations of various antibiotics. Most isolates showed a significant increase in Fosfomycin (7/8), Doxycycline (6/8) and Tetracycline (6/8). Three endodontic isolates showed a significant increase in five of the antibiotics examined at the same time. On exposure to Vancomycin, three endodontic isolates and the two control strains showed an increase. The increase in the ability to form biofilms extended over a concentration range from 1/2 to 1/64 of the MIC concentration. Antibiotics may reach certain niches in the oral cavity at subinhibitory concentrations only. This can increase the biofilm formation by enterococci, and in turn lead to decreased susceptibility of these taxa to antibiotics.
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DOI：
10.2147/ccide.s31005
发表时间：
2013
期刊：
Clinical, cosmetic and investigational dentistry
影响因子：
--
作者：
Do T;Devine D;Marsh PD
通讯作者：
Marsh PD
影响因子：
5
作者：
Arias-Moliz, M. T.;Baca, P.;Osorio, R.
通讯作者：
Osorio, R.
影响因子：
--
作者：
Bernardi, S.;Bianchi, S.;Macchiarelli, G.
通讯作者：
Macchiarelli, G.
影响因子：
3.7
作者：
Anderson AM;Carter KW;Anderson D;Wise MJ
通讯作者：
Wise MJ
影响因子：
5
作者：
Bolfoni, M. R.;Pappen, F. G.;Jacinto, R. C.
通讯作者：
Jacinto, R. C.