Affinity based multilayered polymeric self-assemblies for oral wound applications.
Affinity based multilayered polymeric self-assemblies for oral wound applications.
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DOI：
10.1002/adhm.201200375
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发表时间：
2013-07
影响因子：
10
通讯作者：
Dziubla, Thomas D.
中科院分区：
文献类型：
--
作者：
Authimoolam, Sundar P.;Puleo, David A.;Dziubla, Thomas D.
口腔粘膜炎是一种疼痛和使人衰弱的溃疡性伤口病症，是化疗和/或放疗后经常发生的并发症。虽然目前的治疗标准（例如，凝胶和漱口液）提供暂时的缓解，但仍然需要一种坚固、长效的屏障，其可以在口腔伤口中提供润滑保护，从而增强伤口愈合反应。有人提出，基于亲和性的层-层（LBL）自组装，可以作为一系列的漱口水，可以允许形成保护性屏障，提供一个模块化的方法再生口腔治疗。在这项研究中，生物素化的聚（丙烯酸）的合成开发LBL组件使用生物素-链霉亲和素亲和连接。为了探索所开发的LBL组件潜在地抵抗苛刻的口内环境的能力，进行了体外化学和离体机械测试。稳定性结果证明了显著的LBL屏障稳定性和耐磨性。从统计分析中可以推断，聚合物分子量和LBL层的数量对化学屏障稳定性有显着贡献。此外，生物素缀合的程度对LBL的发展和机械屏障稳定性起关键作用。因此，所提出的基于亲和性的LBL具有优异的屏障性能，为口腔粘膜损伤提供了模块化治疗方法。
Oral mucositis, a painful and debilitating ulcerative wound condition, is a frequently occurring complication following chemo- and/or radiotherapy. While the current standards of therapy (e.g., gels and mouth rinses) provide temporary relief, there is still an unmet need for a robust, long acting barrier that can provide lubricating protection in oral wounds, thereby enhancing the wound healing response. It is proposed that an affinity based layer-by-layer (LBL) self-assembly that can be administered as a series of mouth rinses could permit the formation of protective barriers, providing a modular approach to regenerative oral therapy. In this study, biotinylated poly(acrylic acid) was synthesized for developing LBL assemblies using biotin-streptavidin affinity linkages. To explore the ability of developed LBL assemblies to potentially resist the harsh intraoral environment, in vitro chemical and ex vivo mechanical tests were performed. The stability results demonstrated significant LBL barrier stability with wear resistance. From statistical analyses, it was deduced that polymer MW and the number of LBL layers contributed significantly to chemical barrier stability. Also, the extent of biotin conjugation played a key role for LBL development and in mechanical barrier stability. Thus, the proposed affinity based LBLs with their excellent barrier properties offer a modular treatment approach in oral mucosal injuries.
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DOI：
10.1016/0005-2795(68)90040-8
发表时间：
1968-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
KING, CA
通讯作者：
KING, CA
DOI：
10.1016/s0360-3016(97)00903-6
发表时间：
1998-03-15
影响因子：
7
作者：
Oguchi, M;Shikama, N;Sone, S
通讯作者：
Sone, S
影响因子：
1.8
作者：
ANDRADE, JD;HLADY, V;WEI, AP
通讯作者：
WEI, AP
影响因子：
3.8
作者：
Nam, J-W;Chung, J-W;Kim, Y-K
通讯作者：
Kim, Y-K
影响因子：
9.9
作者：
Jeyachandran, Y. L.;Mielczarski, J. A.;Rai, B.
通讯作者：
Rai, B.