Isolation and molecular characterization of parainfluenza virus 5 in diarrhea-affected piglets in China.

Isolation and molecular characterization of parainfluenza virus 5 in diarrhea-affected piglets in China.
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中国腹泻仔猪中副流感病毒 5 型的分离和分子特征分析

DOI:
10.1292/jvms.17-0581
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发表时间:
2018-04-18
期刊:
The Journal of veterinary medical science
影响因子:
--
通讯作者:
Sun D
Sun D
中科院分区:
其他
文献类型:
--
作者:
Jiang N;Wang E;Guo D;Wang X;Su M;Kong F;Yuan D;Zhai J;Sun D

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最近,副流感病毒5型(PIV 5)感染在哺乳动物中的报道越来越多。本研究从我国4个省、市、自治区的仔猪腹泻病料中分离到5株PIV 5。基于F基因的系统发育树表明,5个分离株与中国小熊猫的PIV 5株ZJQ-221和韩国犬的PIV 5株1168-1密切相关。新分离株在遗传上与其他猪、小牛、恒河猴肾细胞、人和犬PIV 5参考毒株不同。我们的研究揭示了从受感染的仔猪中收集的肠组织样本中存在PIV 5,并提供了有关PIV 5流行病学和组织嗜性的新信息。
Recently, parainfluenza virus 5 (PIV5) infection has been increasingly reported in mammals. In this study, five PIV5 strains were isolated from diarrhea-affected piglets from four provinces or municipalities in China. An F-gene-based phylogenetic tree indicated that the five isolated strains were closely related to the PIV5 strain ZJQ-221 from a lesser panda in China, and the PIV5 strain 1168-1 from a dog in South Korea. The new isolates differed genetically from other pig, calf, rhesus macaque kidney cells, human, and dog PIV5 reference strains. Our study reveals the presence of PIV5 in intestinal tissue samples collected from diarrhea-affected piglets, and provides novel information regarding the epidemiology and tissue tropism of PIV5.
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