Identification and expression profiling of odorant binding proteins and chemosensory proteins between two wingless morphs and a winged morph of the cotton aphid Aphis gossypii glover.
Identification and expression profiling of odorant binding proteins and chemosensory proteins between two wingless morphs and a winged morph of the cotton aphid Aphis gossypii glover.
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DOI：
10.1371/journal.pone.0073524
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhou JJ
中科院分区：
文献类型：
--
作者：
Gu SH;Wu KM;Guo YY;Field LM;Pickett JA;Zhang YJ;Zhou JJ
昆虫与它们的环境相互作用，并利用化学物质对寄主植物条件的变化作出反应。这种生态相互作用是由嗅觉感受器和嗅觉识别蛋白的使用促进的。棉蚜可以根据不同的生态条件改变表型。它们主要以无翅无性形态繁殖，但在温度、植物营养和种群密度等环境因素的影响下，它们会发育出翅膀来寻找配偶或新的植物寄主。两组小的可溶性蛋白，气味结合蛋白（OBPs）和化学感觉蛋白（CSPs）被认为参与了符号化学感知的初始生化识别步骤。然而，这些蛋白质在昆虫嗅觉中发挥的确切分子作用仍有待发现。在这项研究中，我们比较了三个无性发育阶段（无翅的春季和夏季变形和有翼的成年）的转录组，并表征了9个OBP和9个CSP基因。基因结构分析表明，这些基因内含子的数量和长度都要高得多，这似乎是蚜虫OBP和CSP基因的普遍特征。蚜虫的另一个独特特征是CSP转录本的丰度高于OBP转录本，这表明CSP在蚜虫生理和生态学中的重要作用。我们发现，与身体相比，一些转录本在触角中过度表达，而在有翼蚜虫中，与无翼蚜虫相比，这些转录本在有翼蚜虫中高度表达，这表明它们与寄主位置有关。我们检测了这些嗅觉基因在10种蚜虫中的差异表达，并将其表达谱与AphidBase上25个豌豆蚜虫转录组文库的RNA-seq分析结果进行了比较。
Insects interact with their environment and respond to the changes in host plant conditions using semiochemicals. Such ecological interactions are facilitated by the olfactory sensilla and the use of olfactory recognition proteins. The cotton aphid Aphis gossypii can change its phenotype in response to ecological conditions. They reproduce mainly as wingless asexual morphs but develop wings to find mates or new plant hosts under the influence of environmental factors such as temperature, plant nutrition and population density. Two groups of small soluble proteins, odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are believed to be involved in the initial biochemical recognition steps in semiochemical perception. However, the exact molecular roles that these proteins play in insect olfaction remain to be discovered. In this study, we compared the transcriptomes of three asexual developmental stages (wingless spring and summer morphs and winged adults) and characterised 9 OBP and 9 CSP genes. The gene structure analysis showed that the number and length of introns in these genes are much higher and this appears to be unique feature of aphid OBP and CSP genes in general. Another unique feature in aphids is a higher abundance of CSP transcripts than OBP transcripts, suggesting an important role of CSPs in aphid physiology and ecology. We showed that some of the transcripts are overexpressed in the antennae in comparison to the bodies and highly expressed in the winged aphids compared to wingless morphs, suggesting a role in host location. We examined the differential expression of these olfactory genes in ten aphid species and compared the expression profile with the RNA-seq analyses of 25 pea aphid transcriptome libraries hosted on AphidBase.
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DOI：
10.1046/j.1432-1327.1999.00438.x
发表时间：
1999-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Angeli, S;Ceron, F;Pelosi, P
通讯作者：
Pelosi, P
DOI：
10.1016/0020-1790(87)90093-x
发表时间：
1987-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
KLEIN, U
通讯作者：
KLEIN, U
影响因子：
2.3
作者：
Al Abassi, S;Birkett, MA;Woodcock, CM
通讯作者：
Woodcock, CM
影响因子：
2.6
作者：
Ban, L;Scaloni, A;Pelosi, P
通讯作者：
Pelosi, P
影响因子：
3.5
作者：
Nagnan-Le Meillour, P;Cain, AH;Steinbrecht, RA
通讯作者：
Steinbrecht, RA