Tobacco-smoking-related prevalence of methanogens in the oral fluid microbiota.
Tobacco-smoking-related prevalence of methanogens in the oral fluid microbiota.
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DOI：
10.1038/s41598-018-27372-7
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发表时间：
2018-06-15
影响因子：
4.6
通讯作者：
Drancourt M
中科院分区：
文献类型：
--
作者：
Grine G;Terrer E;Boualam MA;Aboudharam G;Chaudet H;Ruimy R;Drancourt M
口腔流体微生物组包括重要的细菌多样性，但迄今为止尚未报道古细菌的存在。为探讨口腔液中是否存在产甲烷古菌（产甲烷菌），采用PCR-测序检测、荧光原位杂交显微观察、分离培养、分子鉴定和基因分型等多相方法对200份口腔液标本中的产甲烷菌进行检测。在存在阴性对照的情况下，64/200（32%）前瞻性分析的口腔液标本为产甲烷菌PCR阳性，均通过测序鉴定为口腔甲烷短杆菌。此外，荧光原位杂交检测到产甲烷菌在19/48（39.6%）的调查标本，形态表明M。口腔链球菌10例，共感染史氏甲烷短杆菌9例。M. 64例PCR阳性标本中46例（71.8%）培养出口腔支原体，PCR阴性标本中无一例培养出口腔支原体;将史密氏菌分离物与M.在一个标本中。多间隔区序列分型发现一个M。oralis基因型，总共有5种不同的基因型，其中19/46（41%）的菌株都属于间隔子4型。统计分析表明，口腔液中的产甲烷菌的PCR检测和吸烟之间存在显着的相关性。这些数据表明M. oralis和M. Smithii是烟草吸烟者中的口腔液体传播的产甲烷菌。这两种产甲烷菌都可以在亲密接触中传播，如母婴接触和接吻。
The oral fluid microbiome comprises an important bacterial diversity, yet the presence of archaea has not been reported so far. In order to quest for the presence of methanogenic archaea (methanogens) in oral fluid, we used a polyphasic approach including PCR-sequencing detection, microscopic observation by fluorescence in-situ hybridization, isolation and culture, molecular identification and genotyping of methanogens in 200 oral fluid specimens. In the presence of negative controls, 64/200 (32%) prospectively analysed oral fluid specimens were PCR-positive for methanogens, all identified as Methanobrevibacter oralis by sequencing. Further, fluorescence in-situ hybridization detected methanogens in 19/48 (39.6%) investigated specimens; with morphology suggesting M. oralis in 10 cases and co-infecting Methanobrevibacter smithii in nine cases. M. oralis was cultured from 46/64 (71.8%) PCR-positive specimens and none of PCR-negative specimens; and one M. smithii isolate was co-cultured with M. oralis in one specimen. Multispacer Sequence Typing found one M. oralis genotype per specimen and a total of five different genotypes with 19/46 (41%) of isolates all belonging to spacer-type four. Statistical analyses showed a significant correlation between the PCR-detection of methanogens in oral fluid and tobacco smoking. These data indicate that M. oralis and M. smithii are oral fluid-borne methanogens in tobacco smokers. Both methanogens could be transmitted during intimate contacts such as mother-to-child contacts and kissing.
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影响因子：
3
作者：
Li, Chao Lun;Jiang, Yun Tao;Shu, Rong
通讯作者：
Shu, Rong
影响因子：
3
作者：
Kozaki, Tomoaki;Hashiguchi, Nobuko;Tochihara, Yutaka
通讯作者：
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DOI：
10.1073/pnas.0812600106
发表时间：
2009-02-17
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
9.4
作者：
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通讯作者：
Drancourt, Michel
影响因子：
2.8
作者：
Luton, PE;Wayne, JM;Riley, PW
通讯作者：
Riley, PW