Cuticular profiling of insecticide resistant Aedes aegypti.
Cuticular profiling of insecticide resistant Aedes aegypti.
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DOI：
10.1038/s41598-023-36926-3
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发表时间：
2023-06-22
影响因子：
4.6
通讯作者：
Casadevall, Arturo
中科院分区：
文献类型：
--
作者：
Jacobs, Ella;Chrissian, Christine;Rankin-Turner, Stephanie;Wear, Maggie;Camacho, Emma;Broderick, Nichole A.;McMeniman, Conor J.;Stark, Ruth E.;Casadevall, Arturo
杀虫剂在减少病媒传播疾病的全球负担方面取得了巨大进展。尽管如此，严重的公共卫生问题仍然存在，因为对杀虫剂具有抗药性的病媒种群继续在全球蔓延。为了避免杀虫剂抗药性，必须了解所有促成机制。基于接触的杀虫剂通过昆虫表皮吸收，所述表皮主要由几丁质多糖、表皮蛋白、烃和酚类生物聚合物、骨质和黑色素组成。表皮界面的改变可以减缓或阻止杀虫剂渗透，这种现象称为表皮阻力。黄热病蚊子，埃及伊蚊，表皮电阻特性是缺乏的。在目前的研究中，我们利用固态核磁共振光谱，气相色谱/质谱和透射电子显微镜，以获得深入了解同类细胞色素P450单加氧酶杀虫剂抗性和敏感的Ae的角质层组成。埃及人。角质层碳氢化合物含量和酚类生物聚合物的沉积没有差异。埃及伊蚊随时间增加并显示出更高的多糖丰度。此外，我们发现这些局部表皮变化与整个蚊子的整体代谢差异相关，这表明在这种主要疾病媒介中存在新的表皮抵抗机制。
Insecticides have made great strides in reducing the global burden of vector-borne disease. Nonetheless, serious public health concerns remain because insecticide-resistant vector populations continue to spread globally. To circumvent insecticide resistance, it is essential to understand all contributing mechanisms. Contact-based insecticides are absorbed through the insect cuticle, which is comprised mainly of chitin polysaccharides, cuticular proteins, hydrocarbons, and phenolic biopolymers sclerotin and melanin. Cuticle interface alterations can slow or prevent insecticide penetration in a phenomenon referred to as cuticular resistance. Cuticular resistance characterization of the yellow fever mosquito, Aedes aegypti, is lacking. In the current study, we utilized solid-state nuclear magnetic resonance spectroscopy, gas chromatography/mass spectrometry, and transmission electron microscopy to gain insights into the cuticle composition of congenic cytochrome P450 monooxygenase insecticide resistant and susceptible Ae. aegypti. No differences in cuticular hydrocarbon content or phenolic biopolymer deposition were found. In contrast, we observed cuticle thickness of insecticide resistant Ae. aegypti increased over time and exhibited higher polysaccharide abundance. Moreover, we found these local cuticular changes correlated with global metabolic differences in the whole mosquito, suggesting the existence of novel cuticular resistance mechanisms in this major disease vector.
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影响因子：
3.8
作者：
Marcombe S;Darriet F;Tolosa M;Agnew P;Duchon S;Etienne M;Yp Tcha MM;Chandre F;Corbel V;Yébakima A
通讯作者：
Yébakima A
影响因子：
5.9
作者：
Adams KL;Sawadogo SP;Nignan C;Niang A;Paton DG;Robert Shaw W;South A;Wang J;Itoe MA;Werling K;Dabiré RK;Diabaté A;Catteruccia F
通讯作者：
Catteruccia F
影响因子：
4.6
作者：
Chang J;Singh J;Kim S;Hockaday WC;Sim C;Kim SJ
通讯作者：
Kim SJ
DOI：
10.3390/molecules22101663
发表时间：
2017-10-05
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Alexandri E;Ahmed R;Siddiqui H;Choudhary MI;Tsiafoulis CG;Gerothanassis IP
通讯作者：
Gerothanassis IP
DOI：
10.1073/pnas.0504982102
发表时间：
2005-08-09
影响因子：
11.1
作者：
Arakane, Y;Muthukrishnan, S;Kramer, KJ
通讯作者：
Kramer, KJ