In vivo assessment of the effects of ginsenoside Rb1 on intimal hyperplasia in ApoE knockout mice.
In vivo assessment of the effects of ginsenoside Rb1 on intimal hyperplasia in ApoE knockout mice.
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DOI：
10.1016/j.jss.2010.01.026
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发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Zhou W
中科院分区：
文献类型：
--
作者：
Chai H;Schultz G;Aghaie K;Zhou W
本研究探讨了人参皂苷Rb1 （Rb1）对ApoE敲除（ApoE−/−）小鼠损伤性内膜增生的影响。我们还研究了超声微图像系统在动态监测管腔直径和流速方面的价值。导丝损伤左颈总动脉远端（CCA）后，ApoE−/−小鼠腹腔注射生理盐水（NS）、同型半胱氨酸（Hcy）、人参皂苷Rb1 （Rb1）或Hcy+Rb1治疗4周。术前和术后每周用超声显微成像系统测量双侧CCA管径和血流速度。在最终超声之后，收集cca并分析内膜-中膜厚度比。与相对稳定的左CCA近端和右CCA相比，超声微成像系统观察各组损伤左CCA远端段管径进行性缩小，血流速度增加。与Rb1组和Rb1+Hcy组相比，NS组和Hcy组的直径缩小程度显著高于Rb1组。术后4周超声检查结果与组织学分析一致。研究表明，Rb1可减轻Hcy对ApoE−/−小鼠颈动脉损伤的影响。该研究还表明，超声微图像系统是监测小鼠模型损伤后腔内缩小的可靠工具。本研究在不牺牲动物的情况下，在小鼠模型中建立了体内监测药物治疗效果的基本步骤。
This study investigated the effects of ginsenoside Rb1 (Rb1) on injury-induced intimal hyperplasia in ApoE knock out (ApoE −/−) mice. We also examined the value of an ultrasound micro-image system in dynamic monitoring of lumen diameter and flow velocity. After guide wire injury of the distal left common carotid artery (CCA), ApoE−/− mice were treated with intraperitoneal infusion of normal saline (NS), Homocysteine (Hcy), ginsenoside Rb1 (Rb1), or Hcy+Rb1 for 4 weeks. Bilateral CCA luminal diameters and flow velocities were measured with an ultrasound micro-image system before surgery and weekly afterwards. Following the final ultrasound, CCAs were harvested and analyzed for intima-medium thickness ratios. Progressive reduction in luminal diameters and increase in flow velocity of the injured left distal CCA segment were observed using ultrasound micro-imaging system in all groups comparing to the relatively stable left proximal CCA and right CCA. The NS and Hcy groups had significant higher degree of diameter reduction comparing to the Rb1 and Rb1+Hcy groups. The ultrasound findings were consistent with histology analyses at 4 weeks post-op. The study suggested that Rb1 attenuated the effects of Hcy on injured carotid arteries of ApoE −/− mice. The study also showed that ultrasound micro-image system was a reliable tool in monitoring luminal reduction after injury in murine model. This study establishes a fundamental step of in vivo monitoring the therapeutic effects of agents in a murine model without sacrificing the animals.
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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发表时间：
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影响因子：
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