Sex and strain differences in renal hemodynamics in mice.
Sex and strain differences in renal hemodynamics in mice.
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DOI：
10.14814/phy2.15644
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发表时间：
2023-03
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
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本研究旨在研究肾脏研究中常用的三种小鼠品系C57 BL 6、129/Sv和C57 BLKS/J小鼠的肾小球滤过率（GFR）和肾血流量（RBF）的性别和品系差异。通过经皮测量清醒小鼠中的FITC-sinitrin清除率来测量GFR。在麻醉状态下，通过放置在肾动脉中的流量探针测量RBF。在C57 BL 6小鼠中，GFR和RBF均无性别差异。在129/Sv小鼠中，雌性在24周龄时的GFR显著高于雄性，但在8周龄时并非如此。然而，男性有较高的RBF和较低的肾血管阻力（RVR）。与129/Sv相似，雌性C57 BLKS/J在8周和24周时的GFR均显著高于雄性，RBF较低，RVR较高。在不同品系中，雄性129/Sv的GFR和RBF均低于雄性C57 BL 6，但与雄性C57 BLKS/J相比，GFR和RBF无显著差异。在C57 BL 6和C57 BLKS/J小鼠之间未观察到GFR或RBF的显著差异。C57 BLKS/J小鼠中eNOS的缺失降低了两种性别的GFR，但降低了雄性的RBF。此外，eNOS−/− dbdb小鼠肾损伤的严重程度没有性别差异。总之，我们的研究表明，小鼠肾脏血流动力学的性别差异是应变和年龄依赖性的。eNOS不参与GFR的性别差异，但参与RBF的性别差异。此外，两性异形并不影响糖尿病肾病肾损伤的严重程度。肾脏血流动力学存在性别和应变差异，这也可能是年龄依赖性的。肾脏血流动力学的性别差异不归因于eNOS，也不影响糖尿病肾病的发展。
The present study was to examine sex and strain differences in glomerular filtration rate (GFR) and renal blood flow (RBF) in C57BL6, 129/Sv, and C57BLKS/J mice, three commonly used mouse strains in renal research. GFR was measured by transdermal measurement of FITC‐sinitrin clearance in conscious mice. RBF was measured by a flow probe placed in the renal artery under an anesthetic state. In C57BL6 mice, there were no sex differences in both GFR and RBF. In 129/Sv mice, females had significantly greater GFR than males at age of 24 weeks, but not at 8 weeks. However, males had higher RBF and lower renal vascular resistance (RVR). Similar to 129/Sv, female C57BLKS/J had significantly greater GFR at both 8 and 24 weeks, lower RBF, and higher RVR than males. Across strains, male 129/Sv had lower GFR and higher RBF than male C57BL6, but no significant difference in GFR and greater RBF than male C57BLKS/J. No significant difference in GFR or RBF was observed between C57BL6 and C57BLKS/J mice. Deletion of eNOS in C57BLKS/J mice reduced GFR in both sexes, but decreased RBF in males. Furthermore, there were no sex differences in the severity of renal injury in eNOS−/− dbdb mice. Taken together, our study suggests that sex differences in renal hemodynamics in mice are strain and age dependent. eNOS was not involved in the sex differences in GFR, but in RBF. Furthermore, the sexual dimorphism did not impact the severity of renal injury in diabetic nephropathy. There are sex and strain differences in renal hemodynamics, which may also be age dependent. The sex difference in renal hemodynamics is not attributed to eNOS, and do not impact the development of diabetic kidney disease.
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DOI：
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C57BL/6 和 129/Sv 小鼠：肾缺血再灌注的遗传差异
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