A selective resonance scattering assay for immunoglobulin G using Cu(II)-ascorbic acid-immunonanogold reaction.
A selective resonance scattering assay for immunoglobulin G using Cu(II)-ascorbic acid-immunonanogold reaction.
复制标题
使用 Cu(II)-抗坏血酸-免疫金反应对免疫球蛋白 G 进行选择性共振散射测定。
DOI:
10.1016/j.ab.2008.06.016
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发表时间:
2008-09
影响因子:
2.9
通讯作者:
中科院分区:
文献类型:
--
作者:
In sodium acetate–acetic acid buffer solution, Au, Ag, Pt, Pd, Fe3O4, and Cu2O nanoparticles have catalytic enhancement effect on the reduction of Cu2+by ascorbic acid to form large copper particles that exhibit a strong resonance scattering peak at 610nm. Those nanocatalytic reactions were studied by the resonance scattering spectral technique, and smaller nanogold exhibited stronger catalytic enhancement effect in pH 4.2 sodium acetate–acetic acid buffer solution. The resonance scattering intensity at 610nm increased linearly with the concentrations of 0.02 to 1.60, 0.040 to 1.20, and 0.12 to 4.70nM nanogold in sizes of 5, 10, and 15nm with detection limits of 0.010, 0.030, and 0.10nM, respectively. An immunonanogold-catalytic resonance scattering bioassay was established, combining the immunonanogold-catalytic effect on CuSO4–ascorbic acid reaction with the resonance scattering detection technique. As a model, 0.03 to 7.5ngml−1immunoglobulin G can be assayed by this immunonanogold-catalytic resonance scattering bioassay with a detection limit of 0.015ngml−1.
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影响因子:
5.4
作者:
Zhiliang Jiang;Yan Li;Shuang-jiao Sun;Bing Chen
通讯作者:
Zhiliang Jiang;Yan Li;Shuang-jiao Sun;Bing Chen
影响因子:
7.4
作者:
Liu, SP;Luo, HQ;Zheng, WX
通讯作者:
Zheng, WX
影响因子:
--
作者:
Z. Demko;K. Sharpless
通讯作者:
Z. Demko;K. Sharpless
影响因子:
1.2
作者:
Aiping Fan;C. Lau;Zhixin Liao;Jianying Liang;Jianzhong Lu
通讯作者:
Aiping Fan;C. Lau;Zhixin Liao;Jianying Liang;Jianzhong Lu
影响因子:
12.6
作者:
Chu, X;Fu, X;Yu, RQ
通讯作者:
Yu, RQ