Age-related characteristics of multipotent human nasal inferior turbinate-derived mesenchymal stem cells.
Age-related characteristics of multipotent human nasal inferior turbinate-derived mesenchymal stem cells.
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DOI：
10.1371/journal.pone.0074330
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sun DI
中科院分区：
文献类型：
--
作者：
Hwang SH;Park SH;Choi J;Lee DC;Oh JH;Yeo UC;Kim SW;Sun DI
多潜能间充质干细胞(MSCs)代表了一种基于细胞的治疗多种退行性疾病的有前景的方法。了解衰老对骨髓间充质干细胞的影响对于自体治疗开发和同种异体捐赠者在老年受试者中都是至关重要的，这些受试者通常患有退行性疾病。在本研究中，我们研究了不同年龄的供者年龄对不同年龄段患者的多潜能人甲状旁腺间充质干细胞(HTMSCs)体外培养的特性、增殖和分化潜能的影响。12名患者包括四个年龄组：(I)20岁，(Ii)20-39岁，(Iii)40-59岁，和(Iv)&60岁。鼻甲部分切除术患者的下鼻甲组织被丢弃。分离hTMSCs后，用流式细胞仪检测hTMSC表面标志CD14、CD19、CD34、CD73、CD90、CD105和人类白细胞抗原DR的表达，并用细胞计数试剂盒(CCK)-8检测细胞增殖情况。通过组织学和成骨细胞基因表达的检测，评价hTMSCs在成骨介质中的分化潜能。流式细胞仪分析显示，hTMSCs CD14、CD19、CD34、HLA-DR阴性，CD73、CD90、CD105阳性，为典型的MSC表型，各年龄组间差异无统计学意义。不同年龄组间hTMSCs的细胞增殖和成骨分化能力也无明显差异。我们得出结论：供者年龄不影响hTMSCs的特性、增殖和成骨分化潜能。在hTMSCs自体或同种异体移植的临床使用指南中，供体年龄可能被排除为一个标准。
Multipotent mesenchymal stem cells (MSCs) represent a promising cell-based therapy for a number of degenerative conditions. Understanding the effect of aging on MSCs is crucial for both autologous therapy development and allogenic donors in older subjects whom degenerative diseases typically afflict. In this study, we investigated the influence of donor age on the characteristics, proliferation, and differentiation potential of in vitro cultures of multipotent human turbinated mesenchymal stem cells (hTMSCs) from patients of various age groups. Twelve patients comprised the four age groups: (I) <20 years, (II) 20–39 years, (III) 40–59 years, and (IV) >60 years. Inferior turbinate tissues were discarded from patients undergoing partial turbinectomy. After isolating hTMSCs, the expression of the hTMSC surface markers CD14, CD19, CD34, CD73, CD90, CD105, and HLA-DR was assessed by FACS analysis, and cell proliferation was assessed using a cell counting kit (CCK)-8. The differentiation potential of hTMSCs was evaluated in osteogenic media by histology and determination of osteoblastic gene expression. FACS analysis revealed that hTMSCs were negative for CD14, CD19, CD34, and HLA-DR, and positive for CD73, CD90, and CD105, representing a characteristic MSC phenotype, and showed no significant differences among the age groups. Cellular proliferation and osteogenic differentiation potential of hTMSCs also showed no significant differences among the age groups. We conclude that donor age does not affect the characteristics, proliferation, and osteogenic differentiation potential of hTMSCs. Donor age may be excluded as a criterion in the guidelines for clinical use of the autologous or allogenic transplantation of hTMSCs.
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影响因子：
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作者：
Kretlow JD;Jin YQ;Liu W;Zhang WJ;Hong TH;Zhou G;Baggett LS;Mikos AG;Cao Y
通讯作者：
Cao Y
影响因子：
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作者：
Huang, Zhinong;Nelson, Ehren Robert;Goodman, Stuart B.
通讯作者：
Goodman, Stuart B.
DOI：
10.1006/bbrc.2001.6270
发表时间：
2002-01-18
影响因子：
3.1
作者：
Erickson, GR;Gimble, JM;Guilak, F
通讯作者：
Guilak, F
影响因子：
1.8
作者：
Chen, D;Zhao, M;Mundy, GR
通讯作者：
Mundy, GR
DOI：
10.2741/s138
发表时间：
2011-01-01
期刊：
Frontiers in bioscience (Scholar edition)
影响因子：
--
作者：
Baek, Wook-Young;Kim, Jung-Eun
通讯作者：
Kim, Jung-Eun