A conduit system distributes chemokines and small blood-borne molecules through the splenic white pulp.
A conduit system distributes chemokines and small blood-borne molecules through the splenic white pulp.
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DOI：
10.1084/jem.20021801
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发表时间：
2003-08-04
期刊：
影响因子：
--
通讯作者：
Mebius RE
中科院分区：
文献类型：
--
作者：
Nolte MA;Beliën JA;Schadee-Eestermans I;Jansen W;Unger WW;van Rooijen N;Kraal G;Mebius RE
脾白色髓仅限于淋巴细胞和树突状细胞。在这里，我们表明，从血液进入这些封闭区域的分子运动也是有限的。大分子如牛血清白蛋白（68 kD）、免疫球蛋白G（150 kD）和500 kD葡聚糖不能进入白色髓，而较小的血液传播分子可直接渗透该隔室。分布局限于间质网络，我们称之为脾管道系统。导管的小管腔含有胶原纤维，并且在T细胞区域中被表达ER-TR 7的网状成纤维细胞包围。它还含有趋化因子CCL 21。相反，在B细胞滤泡中，发现吸引B细胞的趋化因子CXCL 13与ER-TR 7+成纤维细胞的导管和缺乏相关。这些结果显示了网状成纤维细胞的异质性，包裹管道系统，并表明本地产生的趋化因子通过该网状网络运输并呈现在该网状网络上。因此，导管在血液传播和局部产生的分子的分布中起作用，并提供指导淋巴细胞迁移和脾白色髓组织化的框架。
Access to the splenic white pulp is restricted to lymphocytes and dendritic cells. Here we show that movement of molecules from the blood into these confined areas is also limited. Large molecules, such as bovine serum albumin (68 kD), immunoglobulin G (150 kD), and 500 kD dextran are unable to enter the white pulp, whereas smaller blood-borne molecules can directly permeate this compartment. The distribution is restricted to a stromal network that we refer to as the splenic conduit system. The small lumen of the conduit contains collagen fibers and is surrounded in the T cell areas by reticular fibroblasts that express ER-TR7. It also contains the chemokine CCL21. Conversely, in B cell follicles the B cell–attracting chemokine CXCL13 was found to be associated with the conduit and absence of ER-TR7+ fibroblasts. These results show heterogeneity of reticular fibroblasts that enfold the conduit system and suggest that locally produced chemokines are transported through and presented on this reticular network. Therefore, the conduit plays a role in distribution of both blood-borne and locally produced molecules and provides a framework for directing lymphocyte migration and organization of the splenic white pulp.
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影响因子：
15.3
作者：
Ngo, V N;Cornall, R J;Cyster, J G
通讯作者：
Cyster, J G
DOI：
10.1084/jem.192.10.1425
发表时间：
2000-11-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gretz JE;Norbury CC;Anderson AO;Proudfoot AE;Shaw S
通讯作者：
Shaw S
影响因子：
--
作者：
SAITO, H;YOKOI, Y;NAMIHISA, T
通讯作者：
NAMIHISA, T
DOI：
10.1084/jem.193.9.1105
发表时间：
2001-05-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Baekkevold ES;Yamanaka T;Palframan RT;Carlsen HS;Reinholt FP;von Andrian UH;Brandtzaeg P;Haraldsen G
通讯作者：
Haraldsen G
影响因子：
--
作者：
COMPTON, CC;RAVIOLA, E
通讯作者：
RAVIOLA, E