Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing.
Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing.
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DOI：
10.1186/1756-3305-7-332
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发表时间：
2014-07-16
影响因子：
3.2
通讯作者：
Katakura K
中科院分区：
文献类型：
--
作者：
Alam MZ;Bhutto AM;Soomro FR;Baloch JH;Nakao R;Kato H;Schönian G;Uezato H;Hashiguchi Y;Katakura K
皮肤利什曼病（CL）是一个主要的和快速增长的公共卫生问题，无论是在当地的巴基斯坦人口和难民营中的阿富汗难民。主要利什曼原虫是巴基斯坦CL的病原体之一。对66份L.（L.）主要分离自CL患者的皮肤活检。采用10个独立的L.（L.）major的遗传多态性和群体结构。（L.）从CL人类病例中分离的主要DNA。将它们的微卫星图谱与130个已分型的L.（L.）主要来自不同的地理区域。所有标记均表现出多态性，在66个苜蓿品种中共检测到51个MLMT谱。（L.）主要的DNA样本数据显示显着的微卫星多态性与罕见的等位基因杂合性。基于贝叶斯模型的方法和系统发育分析推断两个L。（L.）巴基斯坦的主要人口。34个样品属于一个群体，其余32 L。（L.）主要样本聚集到另一个种群中。两个巴基斯坦L。（L.）主要群体形成独立的集群，这与L.（L.）主要来自中亚、伊朗、中东和非洲。L.（L.）主要可能与巴基斯坦信德省不同种类的白蛉和/或啮齿类宿主的存在有关。因此，有必要对CL的流行病学进行全面研究，包括这些疫源地中的水库和白蛉媒介的情况或传播。
Cutaneous leishmaniasis (CL) is a major and fast increasing public health problem, both among the local Pakistani populations and the Afghan refugees in camps. Leishmania (Leishmania) major is one of the etiological agents responsible for CL in Pakistan. Genetic variability and population structure have been investigated for 66 DNA samples of L. (L.) major isolated from skin biopsy of CL patients. Multilocus microsatellite typing (MLMT), employing 10 independent genetic markers specific to L. (L.) major, was used to investigate the genetic polymorphisms and population structures of Pakistani L. (L.) major DNA isolated from CL human cases. Their microsatellite profiles were compared to those of 130 previously typed strains of L. (L.) major from various geographical localities. All the markers were polymorphic and fifty-one MLMT profiles were recognized among the 66 L. (L.) major DNA samples. The data displayed significant microsatellite polymorphisms with rare allelic heterozygosities. A Bayesian model-based approach and phylogenetic analysis inferred two L. (L.) major populations in Pakistan. Thirty-four samples belonged to one population and the remaining 32 L. (L.) major samples grouped together into another population. The two Pakistani L. (L.) major populations formed separate clusters, which differ genetically from the populations of L. (L.) major from Central Asia, Iran, Middle East and Africa. The considerable genetic variability of L. (L.) major might be related to the existence of different species of sand fly and/or rodent reservoir host in Sindh province, Pakistan. A comprehensive study of the epidemiology of CL including the situation or spreading of reservoirs and sand fly vectors in these foci is, therefore, warranted.
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影响因子：
3.4
作者：
Al-Jawabreh, Amer;Diezmann, Stephanie;Schoenian, Gabriele
通讯作者：
Schoenian, Gabriele
影响因子：
3.2
作者：
Botilde, Yanick;Laurent, Thierry;Dujardin, Jean-Claude
通讯作者：
Dujardin, Jean-Claude
影响因子：
9.5
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M
影响因子：
3.2
作者：
Alam, Mohammad Zahangir;Kuhls, Katrin;Schoenian, Gabriele
通讯作者：
Schoenian, Gabriele
影响因子：
1.9
作者：
Hamarsheh, O.;Presber, W.;Schoenian, G.
通讯作者：
Schoenian, G.