Intracranial electrophysiological recordings on a swine model of mesial temporal lobe epilepsy.
Intracranial electrophysiological recordings on a swine model of mesial temporal lobe epilepsy.
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DOI：
10.3389/fneur.2023.1077702
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发表时间：
2023
影响因子：
3.4
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中科院分区：
文献类型：
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测试实验室猪急性癫痫持续状态模型颅内电生理记录的可行性和可靠性。对 17 头体重在 25 至 35 kg 的雄性巴马猪 (Sus scrofa Domestica) 进行海马内注射红藻氨酸 (KA)。两个共 16 个通道的立体脑电图 (SEEG) 电极沿着感觉运动皮层两侧植入到海马体。每天记录 2 小时脑电活动，持续 9-28 天。测试了三种 KA 剂量以评估能够引起癫痫持续状态的量。记录并比较 KA 注射前后的局部场电位 (LFP)。我们对 KA 注射后 4 周内的癫痫模式进行了量化，包括发作间期尖峰、癫痫发作和高频振荡 (HFO)。使用组内相关系数 (ICC) 对发作间期 HFO 率进行重测可靠性，以评估该模型的记录稳定性。 KA剂量试验表明，海马内注射10μl（1.0μg/μl）可成功诱发持续4至12小时的癫痫持续状态。在该剂量下，8头猪（占总数的50%）在视频SEEG记录期的后4周内出现了长期癫痫事件（强直性慢性癫痫发作 + 发作间期峰值n = 5，仅发作间期峰值n = 3）。四头猪（占总数的 25%）没有癫痫活动，另外四头猪（25%）失去了帽子或没有完成实验。显示癫痫样事件的动物被分组为E +（n = 8），而没有显示癫痫事件迹象的四只动物被分组为E-（n = 4）。在 KA 后 4 周内，4 只 E + 动物总共捕获了 46 次电生理癫痫发作，最早发作于第 9 天。癫痫发作持续时间为 12 至 45 秒。与基线相比，在 KA 后期间（第 1、2、4 周，p<<0.05），E+ 组中观察到海马 HFO 率（数量/分钟）显着增加。但与基线率相比，E-显示没有变化或下降（第 2 周，p = 0.43）。组间比较显示，E + 组的 HFO 率远高于 E-组（F = 35，p < 0.01）。从 HFO 率量化的高 ICC 值 [ICC (1, k) = 0.81, p < 0.05] 表明该模型在 KA 后 4 周内对 HFO 进行了稳定的测量。本研究测量了 KA 诱发的内侧颞叶癫痫 (mTLE) 猪模型的颅内电生理活动。使用临床 SEEG 电极，我们区分了猪脑中的异常脑电图模式。 KA 后时期 HFO 率的高重测可靠性表明该模型可用于研究癫痫发生机制。使用猪可以为临床癫痫研究提供令人满意的转化价值。
To test the feasibility and reliability of intracranial electrophysiological recordings in an acute status epilepticus model on laboratory swine. Intrahippocampal injection of kainic acid (KA) was performed on 17 male Bama pigs (Sus scrofa domestica) weighing between 25 and 35 kg. Two stereoelectroencephalography (SEEG) electrodes with a total of 16 channels were implanted bilaterally along the sensorimotor cortex to the hippocampus. Brain electrical activity was recorded 2 h daily for 9–28 days. Three KA dosages were tested to evaluate the quantities capable of evoking status epilepticus. Local field potentials (LFPs) were recorded and compared before and after the KA injection. We quantified the epileptic patterns, including the interictal spikes, seizures, and high-frequency oscillations (HFOs), up to 4 weeks after the KA injection. Test–retest reliability using intraclass correlation coefficients (ICCs) were performed on interictal HFO rates to evaluate the recording stability of this model. The KA dosage test suggested that a 10 μl (1.0 μg/μl) intrahippocampal injection could successfully evoke status epilepticus lasting from 4 to 12 h. At this dosage, eight pigs (50% of total) had prolonged epileptic events (tonic-chronic seizures + interictal spikes n = 5, interictal spikes alone n = 3) in the later 4 weeks of the video-SEEG recording period. Four pigs (25% of total) had no epileptic activities, and another four (25%) had lost the cap or did not complete the experiments. Animals that showed epileptiform events were grouped as E + (n = 8) and the four animals showing no signs of epileptic events were grouped as E– (n = 4). A total of 46 electrophysiological seizures were captured in the 4-week post-KA period from 4 E + animals, with the earliest onset on day 9. The seizure durations ranged from 12 to 45 s. A significant increase of hippocampal HFOs rate (num/min) was observed in the E+ group during the post-KA period (weeks 1, 2,4, p < 0.05) compared to the baseline. But the E-showed no change or a decrease (in week 2, p = 0.43) compared to their baseline rate. The between-group comparison showed much higher HFO rates in E + vs. E – (F = 35, p < 0.01). The high ICC value [ICC (1, k) = 0.81, p < 0.05] quantified from the HFO rate suggested that this model had a stable measurement of HFOs during the four-week post-KA periods. This study measured intracranial electrophysiological activity in a swine model of KA-induced mesial temporal lobe epilepsy (mTLE). Using the clinical SEEG electrode, we distinguished abnormal EEG patterns in the swine brain. The high test–retest reliability of HFO rates in the post-KA period suggests the utility of this model for studying mechanisms of epileptogenesis. The use of swine may provide satisfactory translational value for clinical epilepsy research.
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影响因子：
5.3
作者：
Cimbalnik J;Brinkmann B;Kremen V;Jurak P;Berry B;Gompel JV;Stead M;Worrell G
通讯作者：
Worrell G
影响因子：
5.6
作者：
Carriero, Giovanni;Arcieri, Salvatore;de Curtis, Marco
通讯作者：
de Curtis, Marco
影响因子：
5.6
作者：
Frauscher B;Bartolomei F;Kobayashi K;Cimbalnik J;van 't Klooster MA;Rampp S;Otsubo H;Höller Y;Wu JY;Asano E;Engel J Jr;Kahane P;Jacobs J;Gotman J
通讯作者：
Gotman J
影响因子：
5.6
作者：
Jiruska P;Alvarado-Rojas C;Schevon CA;Staba R;Stacey W;Wendling F;Avoli M
通讯作者：
Avoli M
影响因子：
6.7
作者：
Buzsáki G;Silva FL
通讯作者：
Silva FL