The response of dark septate endophytes (DSE) to heavy metals in pure culture.
The response of dark septate endophytes (DSE) to heavy metals in pure culture.
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纯培养物中深色有隔内生菌（DSE）对重金属的反应。
DOI：
10.1371/journal.pone.0047968
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Ban Y;Tang M;Chen H;Xu Z;Zhang H;Yang Y
暗隔膜内生菌（DSE）广泛存在于重金属胁迫下的植物中。然而，很少有人知道DSE暴露于重金属的反应。从沙打旺（Astragalus adsurgens Pall.）我国铅锌尾矿上的苗木。根据形态特征和DNA序列分析，将分离菌株鉴定为柱孢顶囊壳菌（Gaeumannomycescylindrosporus）、类茎点霉（Paraphomachelicola）、瓶霉（Phialophoramustea）、鲑外瓶霉（Exophialasalmonis）和枝孢枝孢霉（Cladosporiumcladosporioides）。G.柱孢霉（Cylindrosporus）对Pb胁迫的响应。扫描电镜观察G.在固体培养基上生长的Cylindrosporus表现出对Pb的响应，菌丝卷曲，形成菌丝线圈。相反，在液体培养基中，菌丝变厚和肿胀与铅（II）浓度的增加。我们解释这些变化与细胞壁成分的变化有关。我们还表明，真菌黑色素含量增加，此外Pb（II）。黑色素作为细胞壁的重要组成部分，是一种重要的抗氧化剂，可以降低重金属的毒性。同时测定了G.结果表明，随着Pb（II）浓度的增加，两种细菌的生长速率先增大后减小。抗氧化酶活性的测定结果表明，超氧化物歧化酶（SOD）活性与Pb（II）浓度呈显著正相关（r = 0.957，P<0.001）。 总之，我们的观察表明，细胞内的抗氧化系统，特别是真菌黑色素，在减轻重金属的危害中发挥着重要作用。
Dark septate endophytes (DSE) occur widely in association with plants exposed to heavy metal stress. However, little is known about the response of DSE exposed to heavy metals. In this study, five DSE were isolated from the roots of Astragalus adsurgens Pall. seedlings growing on lead-zinc mine tailings in China. Based on morphological characteristics and DNA sequence analyses, the isolates were identified as Gaeumannomyces cylindrosporus, Paraphoma chrysanthemicola, Phialophora mustea, Exophiala salmonis, and Cladosporium cladosporioides. G. cylindrosporus was selected to explore responses to Pb stress. Scanning electron microscopic observations of G. cylindrosporus grown on solid medium revealed curling of hyphae and formation of hyphal coils in response to Pb. In contrast, in liquid medium, hyphae became thick and swollen with an increase in Pb (II) concentration. We interpret that these changes are related to the variation in cell wall components. We also demonstrated that fungal melanin content increased with the addition of Pb(II). Melanin, as an important component in the cell wall, is known to be an essential antioxidant responsible for decreasing heavy metal toxicity. We also measured the total soluble protein content and glutathione (GSH) concentrations in G. cylindrosporus and found that they initially increased and then decreased with the increase of Pb(II) concentrations. The antioxidant enzyme activities were also examined, and the results showed that superoxide dismutase (SOD) activity was significantly positively correlated with Pb(II) concentrations (r = 0.957, P<0.001). Collectively, our observations indicate that the intracellular antioxidant systems, especially fungal melanin, play an important role in abating the hazards of heavy metals.
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影响因子：
11.8
作者：
Granero, S;Domingo, JL
通讯作者：
Domingo, JL
影响因子：
2.1
作者：
Gadd, GM;Ramsay, L;Ritz, K
通讯作者：
Ritz, K
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2.8
作者：
ELLIS, DH;GRIFFITHS, DA
通讯作者：
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3.4
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Tomaro, ML