Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects.
Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects.
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DOI：
10.1038/s41598-017-13326-y
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发表时间：
2017-10-11
影响因子：
4.6
通讯作者：
Ko CC
中科院分区：
文献类型：
--
作者：
Lee DJ;Lee YT;Zou R;Daniel R;Ko CC
羟基磷灰石-胶原（HC）复合材料可以模拟天然骨的组成和超微结构，并提供足够的生物活性材料-组织相互作用。多巴胺（DA）的掺入是增加HC材料的机械强度以接近皮质骨的机械强度的关键之一。在这项研究中，在体外培养大鼠骨髓间充质干细胞（rMSCs）的聚多巴胺-lacedhydroxyapatite胶原硅酸钙（HCCS-PDA）和HCCS涂层板上的成骨作用进行了检查。活/死细胞毒性试验显示HCCS-PDA组的细胞毒性较低，体外细胞粘附、增殖和矿化能力均高于HCCS组。对于体内骨再生，将具有或不具有rMSC聚集体的HCCS-PDA或HCCS颗粒植入大鼠临界尺寸的颅骨缺损（CSD）中。12周后，通过放射学、组织学和组织形态计量学评价颅骨骨再生。虽然大多数新骨形成发生在具有rMSC聚集体的HCCS-PDA颗粒周围，但不具有rMSC聚集体的HCCS-PDA颗粒显示出有限的骨传导性。有或没有rMSC聚集体的HCCS导致较少的骨形成，表明DA在有效的骨再生中的突出作用。因此，HCCS-PDA生物材料与rMSCs的帮助下，可用于开发治疗策略，在骨组织工程的临床应用。
A hydroxyapatite-collagen (HC) composite material can mimic composition and ultra-structures of natural bone and provide adequate bioactive material-tissue interactions. Incorporation of dopamine (DA) is one of keys in increasing the mechanical strength of the HC material to approaching that of cortical bone. In this study, the in vitro osteogenic effects of polydopamine-laced hydroxyapatite collagen calcium silicate (HCCS-PDA) were examined by culturing rat mesenchymal stem cells (rMSCs) on HCCS-PDA and HCCS coated plates. HCCS-PDA group demonstrated less cytotoxic from Live/Dead cytotoxic assay and displayed higher cell attachment, proliferation and mineralization than the HCCS group in vitro. For in vivo bone regeneration, HCCS-PDA or HCCS particulates with or without rMSC aggregates were implanted into rat critical-sized calvarial defects (CSD). After 12 weeks, calvarial bone regeneration was evaluated radiographically, histologically, and histomorphometrically. While the majority of new bone formation occurred around the HCCS-PDA particulates with rMSC aggregates, The HCCS-PDA particulates without rMSC aggregates showed limited osteoconductivity. HCCS with or without rMSC aggregates resulted in less bone formation, indicating a prominent role of DA in effective bone regeneration. Therefore, the HCCS-PDA biomaterial with the aid of rMSCs can be used to develop therapeutic strategies in bone tissue engineering with numerable clinical applications.
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