Morphological and chemical characterization of bonding hydrophobic adhesive to dentin using ethanol wet bonding technique.
Morphological and chemical characterization of bonding hydrophobic adhesive to dentin using ethanol wet bonding technique.
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DOI：
10.1016/j.dental.2009.03.006
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发表时间：
2009-08
期刊：
影响因子：
5
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Shin, T. Phillip;Yao, Xiaomei;Huenergardt, Robin;Walker, Mary P.;Wang, Yong
BisGMA是一种广泛用于牙本质粘合剂的组分，在固化后具有非常好的机械性能，但相对疏水，因此不能充分渗透水润湿的脱矿牙本质胶原。开发技术，将导致最佳的渗透到脱矿牙本质基质的疏水成分是非常重要的。本研究的目的是评估界面的形态和化学特性时，所得的胶粘剂-牙本质界面的乙醇湿粘接技术与疏水胶粘剂使用。从六颗未萌出的人类第三磨牙中取出三分之一的牙冠;用600粒度的SiC创建均匀的涂抹层。牙本质表面用35%磷酸蚀刻15秒，然后使用水湿或乙醇湿粘合技术施加BisGMA/HEMA模型粘合剂。将粘接剂/牙本质界面标本切取5 μ m厚切片，Goldner三色染色，进行光学显微镜观察。用扫描电镜和显微拉曼光谱分析了伴生板坯。乙醇的存在下，在脱矿牙本质增加粘附胶原包封所示的三色染色。SEM结果表明，乙醇湿键合改善了界面质量。显微拉曼光谱分析的牙本质/粘合剂界面表明，有一个逐渐减少的渗透试样使用水湿粘结的BisGMA组件，而相对均匀的分布的疏水性BisGMA组件注意到在与乙醇湿粘结的界面。用乙醇代替水进行湿粘合允许更好的BisGMA渗透，改善界面质量。我们推测，从使用乙醇湿粘合的样本中观察到的疏水性BisGMA的更高渗透和更好的胶原蛋白包封将导致更持久的粘合，因为对水解攻击的抗性提高。
BisGMA, a widely used component in dentin adhesive has very good mechanical properties after curing, but is relatively hydrophobic and thus, does not adequately infiltrate the water wet demineralized dentin collagen. Developing techniques that would lead to optimum infiltration of the hydrophobic component into the demineralized dentin matrix is very important. The purpose of this study was to evaluate interfacial morphological and chemical characteristics of the resultant adhesive-dentin interface when the ethanol wet bonding technique is used with hydrophobic adhesives. The occlusal one-third of the crown was removed from six unerupted human third molars; a uniform smear layer was created with 600 grit SiC. The dentin surface was etched with 35% phosphoric acid for 15 seconds before applying BisGMA/HEMA model adhesive using either water wet or ethanol wet bonding technique. Five-micro-thick sections of adhesive/dentin interface specimens were cut and stained with Goldner’s trichrome for light microscopy. Companion slabs were analyzed with SEM and micro-Raman spectroscopy. The presence of ethanol in the demineralized dentin increased adhesive collagen encapsulation as indicated by trichrome staining. The SEM results confirmed that the ethanol wet bonding improved the quality of the interface. Micro-Raman spectral analysis of the dentin/adhesive interface indicated there was a gradual decrease in penetration of BisGMA component for specimens using water wet bonding, while relatively homogeneous distribution of the hydrophobic BisGMA component was noted in the interface with ethanol wet bonding. Wet bonding with ethanol instead of water permits better BisGMA infiltration improving the quality of interface. We speculate that the higher infiltration of hydrophobic BisGMA and better collagen encapsulation observed from the specimens using ethanol wet bonding would lead to more durable bonds because of improved resistance to hydrolytic attack.
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