A PSTOL-like gene, TaPSTOL, controls a number of agronomically important traits in wheat.
A PSTOL-like gene, TaPSTOL, controls a number of agronomically important traits in wheat.
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DOI：
10.1186/s12870-018-1331-4
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发表时间：
2018-06-08
影响因子：
5.3
通讯作者：
Wallington EJ
中科院分区：
文献类型：
--
作者：
Milner MJ;Howells RM;Craze M;Bowden S;Graham N;Wallington EJ
磷 (P) 是植物生长必需的大量营养素，优良品种的作物需要大量磷来维持产量。全球约70%的耕地缺磷，最近估计全球磷资源将在本世纪末耗尽，这增加了对更有效利用磷的作物的需求。因此，育种者和农民都非常希望更好地了解植物如何能够在较低的磷投入下保持产量。在这里，我们克隆了水稻 PSTOL 基因 (OsPSTOL) 的小麦 (Triticum aestivum L.) 同源物，并表征了其在磷酸盐营养和其他农艺重要性状中的作用。 TaPSTOL 是位于 5A 染色体短臂上的单拷贝基因，编码假定的激酶蛋白，与 OsPSTOL 具有高度的序列相似性。我们对 24 个不同小麦品种和 (3) 三个硬粒小麦品种的 TaPSTOL 进行了重新测序。在 26 个种质中未检测到序列差异，而在其中一个硬粒小麦的启动子区域中发现了两个插入缺失。我们对不同磷浓度下 TaPSTOL 的表达进行了表征，并证明在磷限制条件下，启动子在根尖和毛发中被诱导。 TaPSTOL 在转基因小麦品系中的过表达和 RNAi 沉默表明，对根生物量、与磷处理无关的开花时间、分蘖数和种子产量有显着影响，与 TaPSTOL 的表达相关。然而，这并没有增加 PUE，因为谷物中 P 浓度的升高并不对应于产量的增加。对小麦中 TaPSTOL 表达的操作表明，除了产量之外，TaPSTOL 与 OsPSTOL 一样具有许多先前描述的表型优势。此外，我们表明 TaPSTOL 有助于其他重要的农艺性状，包括开花时间和籽粒大小。对广泛选择的小麦品种（涵盖英国面包小麦 91% 的遗传多样性）的 TaPSTOL 序列进行的分析表明，该基因的遗传变异非常小，这表明该位点可能处于高选择压力下。本文的在线版本 (10.1186/s12870-018-1331-4) 包含补充材料，可供授权用户使用。
Phosphorus (P) is an essential macronutrient for plant growth, and is required in large quantities by elite varieties of crops to maintain yields. Approximately 70% of global cultivated land suffers from P deficiency, and it has recently been estimated that worldwide P resources will be exhausted by the end of this century, increasing the demand for crops more efficient in their P usage. A greater understanding of how plants are able to maintain yield with lower P inputs is, therefore, highly desirable to both breeders and farmers. Here, we clone the wheat (Triticum aestivum L.) homologue of the rice PSTOL gene (OsPSTOL), and characterize its role in phosphate nutrition plus other agronomically important traits. TaPSTOL is a single copy gene located on the short arm of chromosome 5A, encoding a putative kinase protein, and shares a high level of sequence similarity to OsPSTOL. We re-sequenced TaPSTOL from 24 different wheat accessions and (3) three T. durum varieties. No sequence differences were detected in 26 of the accessions, whereas two indels were identified in the promoter region of one of the durum wheats. We characterised the expression of TaPSTOL under different P concentrations and demonstrated that the promoter was induced in root tips and hairs under P limiting conditions. Overexpression and RNAi silencing of TaPSTOL in transgenic wheat lines showed that there was a significant effect upon root biomass, flowering time independent of P treatment, tiller number and seed yield, correlating with the expression of TaPSTOL. However this did not increase PUE as elevated P concentration in the grain did not correspond to increased yields. Manipulation of TaPSTOL expression in wheat shows it is responsible for many of the previously described phenotypic advantages as OsPSTOL except yield. Furthermore, we show TaPSTOL contributes to additional agronomically important traits including flowering time and grain size. Analysis of TaPSTOL sequences from a broad selection of wheat varieties, encompassing 91% of the genetic diversity in UK bread wheat, showed that there is very little genetic variation in this gene, which would suggest that this locus may have been under high selection pressure. The online version of this article (10.1186/s12870-018-1331-4) contains supplementary material, which is available to authorized users.
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DOI：
10.1534/g3.114.012963
发表时间：
2014-09-18
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Mackay IJ;Bansept-Basler P;Barber T;Bentley AR;Cockram J;Gosman N;Greenland AJ;Horsnell R;Howells R;O'Sullivan DM;Rose GA;Howell PJ
通讯作者：
Howell PJ
影响因子：
14.9
作者：
Nakagawa S;Niimura Y;Gojobori T;Tanaka H;Miura K
通讯作者：
Miura K
影响因子：
3.3
作者：
Qi, LL;Echalier, B;Gill, BS
通讯作者：
Gill, BS
影响因子：
6.9
作者：
Hammond, John P.;Broadley, Martin R.;Greenwood, Duncan J.
通讯作者：
Greenwood, Duncan J.
影响因子：
5.4
作者：
Ni, JJ;Wu, P;Huang, N
通讯作者：
Huang, N