Early life supply of competitive exclusion products reduces colonization of extended spectrum beta-lactamase-producing Escherichia coli in broilers.
Early life supply of competitive exclusion products reduces colonization of extended spectrum beta-lactamase-producing Escherichia coli in broilers.
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DOI：
10.1016/j.psj.2020.04.025
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发表时间：
2020-08
期刊：
影响因子：
4.4
通讯作者：
Stegeman A
中科院分区：
文献类型：
--
作者：
Dame-Korevaar A;Fischer EAJ;van der Goot J;Velkers F;Ceccarelli D;Mevius D;Stegeman A
肉鸡是产超广谱β-内酰胺酶（ESBL/pAmpC）细菌的重要宿主。在先前的研究中，在用高剂量产ESBL/pAmpC大肠埃希菌进行挑战之前，单一供应竞争性排除（CE）产品导致定植、排泄和传播减少，但不能防止定植。假设机制是竞争，因此，在本研究中，长期供应CE产品对产ESBL E.在第0天或第5天用低剂量攻毒后，研究了大肠杆菌。将1日龄肉鸡（Ross 308）（n = 220）圈养在隔离器中。从第0天到第14天，在饮用水中提供两种CE产品，其中含有微生物发酵的肠道细菌（CEP）或一系列益生元和益生菌（SYN）。在第0天或第5天，用0.5 mL含101或102 cfu/mL E.将编码β-内酰胺酶基因blaCTX-M-1的大肠杆菌（CTX-M-1-E.大肠杆菌）。CTX-M-1-E的存在和浓度。使用泄殖腔拭子（第0-14、16、19和21天）和盲肠内容物（第21天）测定大肠杆菌。采用考克斯比例风险模型和混合线性回归模型确定干预对定植和排泄的影响（log 10 cfu/g）。在孵化当天攻毒时，未观察到CEP的影响。当在第5天攻击时，CEP和SYN都导致CTX-M-1-E对定殖的预防。大肠杆菌。在其余的隔离者中，我们观察到定植时间（风险比在3.71 × 10−3和3.11之间）、排泄时间（高达−1.60 log 10 cfu/g）和盲肠内容物（高达−2.80 log 10 cfu/g）缩短，传播率降低1.5至3倍。产ESBL大肠杆菌低剂量攻毒后定植。大肠杆菌感染可通过CE产品预防。然而，如果至少有一只鸟被殖民化，它就会在整个鸟群中传播。在孵化后不久提供的CE产品的长期供应可能适用于作为一种干预措施，以减少肉鸡生产链中产ESBL/pAmpC细菌的流行。
Broilers are an important reservoir of extended spectrum beta-lactamase and AmpC beta-lactamase (ESBL/pAmpC)-producing bacteria. In previous studies, a single supply of a competitive exclusion (CE) product before challenge with a high dose of ESBL/pAmpC-producing Escherichia coli led to reduced colonization, excretion, and transmission, but could not prevent colonization. The hypothesized mechanism is competition; therefore, in this study the effect of a prolonged supply of CE products on colonization, excretion, and transmission of ESBL-producing E. coli after challenge with a low dose at day 0 or day 5 was investigated. Day-old broilers (Ross 308) (n = 220) were housed in isolators. Two CE products, containing unselected fermented intestinal bacteria (CEP) or a selection of pre- and probiotics (SYN), were supplied in drinking water from day 0 to 14. At day 0 or 5, broilers were challenged with 0.5 mL with 101 or 102 cfu/mL E. coli encoding the beta-lactamase gene blaCTX-M-1 on an IncI plasmid (CTX-M-1-E. coli). Presence and concentration of CTX-M-1-E. coli were determined using cloacal swabs (days 0–14, 16, 19, and 21) and cecal content (day 21). Cox proportional hazard model and a mixed linear regression model were used to determine the effect of the intervention on colonization and excretion (log10 cfu/g). When challenged on the day of hatch, no effect of CEP was observed. When challenged at day 5, both CEP and SYN led to a prevention of colonization with CTX-M-1-E. coli in some isolators. In the remaining isolators, we observed reduced time until colonization (hazard ratio between 3.71 × 10−3 and 3.11), excretion (up to −1.60 log10 cfu/g), and cecal content (up to −2.80 log10 cfu/g), and a 1.5 to 3-fold reduction in transmission rate. Colonization after a low-dose challenge with ESBL-producing E. coli can be prevented by CE products. However, if at least 1 bird is colonized it spreads through the whole flock. Prolonged supply of CE products, provided shortly after hatch, may be applicable as an intervention to reduce the prevalence of ESBL/pAmpC-producing bacteria in the broiler production chain.
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影响因子：
1.4
作者：
Sung, HW;Reddy, SM;Fadly, AM
通讯作者：
Fadly, AM
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