The Expression of Invasive Behavior of Differentiated Squamous Carcinoma Cell Line Evaluated by an in vitro Invasion Model
The Expression of Invasive Behavior of Differentiated Squamous Carcinoma Cell Line Evaluated by an in vitro Invasion Model
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体外侵袭模型评价分化鳞状癌细胞系侵袭行为的表达
DOI：
10.1111/j.1349-7006.1993.tb00151.x
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
E. Yamamoto
中科院分区：
文献类型：
--
作者：
E. Kawahara;Y. Okada;I. Nakanishi;K. Iwata;S. Kojima;S. Kumagai;E. Yamamoto
为了阐明影响口腔鳞状细胞癌侵袭性表达的因素，我们对在3T3细胞包埋的胶原凝胶上培养的高分化鳞状癌细胞系OSC-19（口腔鳞状细胞癌）和未分化癌细胞系KB（体外侵袭模型）进行生化和形态学比较。 OSC-19细胞侵入3T3细胞包埋的胶原凝胶，而KB细胞和3T3无细胞凝胶基质上的OSC-19细胞侵入性较小。与KB细胞相比，培养的OSC-19细胞具有较低的增殖活性、较低的层粘连蛋白分泌和较高的纤连蛋白分泌。虽然形成了层粘连蛋白和IV型胶原沉积的基底膜，但在侵袭前沿处是不连续的。明胶酶谱和蛋白质印迹显示基质金属蛋白酶 (MMP)，即 72 kDa 明胶酶 (MMP-2) 和 92 kDa 明胶酶 (MMP-9)。检测了明胶分解活性，OSC-19 细胞中的明胶分解活性高于 KB 细胞或 3T3 无细胞模型的 OSC-19 细胞。通过免疫组织化学分析，发现 MMP-2 阳性细胞分散在两种细胞系中，没有任何优先定位，MMP-9 阳性细胞位于 OSC-19 细胞的侵袭前沿。这些结果强烈表明，鳞状细胞癌的侵袭性与纤连蛋白的细胞基质粘附以及金属蛋白酶（尤其是 MMP-9）的局部加工密切相关，MMP-9 在降解细胞外基质成分中发挥着主要作用。
In order to elucidate the factors contributory to the expression of invasiveness of oral squamous cell carcinoma, we conducted biochemical and morphological comparisons of well differentiated squamous carcinoma cell line OSC‐19 (oral squamous cell carcinoma) and undifferentiated carcinoma cell line KB, both cultured on 3T3 cell‐embedded collagen gel (in vitro invasion model). OSC‐19 cells invaded 3T3 cell‐embedded collagen gel, while KB cells and OSC‐19 cells on 3T3 cell‐free gel matrix were less invasive. Cultured OSC‐19 cells were characterized by lower proliferating activity, lower secretion of laminin and higher secretion of fibronectin than those of KB cells. Although the basement membrane with deposition of laminin and type IV collagen was formed, it was discontinuous at the invasion front. Gelatin zymography and western blotting showed matrix metalloproteinases (MMP), i.e., 72 kDa gelatinase (MMP‐2) and 92 kDa gelatinase (MMP‐9). Gelatinolytic activity was assayed, and was higher in OSC‐19 cells than in KB cells or OSC‐19 cells of the 3T3 cell‐free model. By immuno‐histochemical analysis, MMP‐2‐positive cells were found scattered in both cell lines without any preferential localization, and the positivity for MMP‐9 was localized in the invasion front of OSC‐19 cells. These results strongly suggest that the invasiveness of squamous cell carcinoma is well correlated with cell‐matrix adhesion by fibronectin and with focal elaboration of metalloproteinases, especially MMP‐9, which play a major role in degrading the extracellular matrix components.
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影响因子：
27.4
作者：
OKADA, Y;TAKEUCHI, N;NAGASE, H
通讯作者：
NAGASE, H
影响因子：
3.9
作者：
Ennouri, Marwa;Petit, Laeticia;Elhouichet, Habib
通讯作者：
Elhouichet, Habib
微管中 GTP 水解伴随的结构变化：来自缓慢水解类似物鸟苷基-(α,β)-亚甲基二膦酸酯的信息。
DOI：
10.1083/jcb.128.1.117
发表时间：
1995-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hyman AA;Chrétien D;Arnal I;Wade RH
通讯作者：
Wade RH
人体皮肤中纤连蛋白、胶原蛋白和层粘连蛋白整合素受体的定位。
DOI：
10.1172/jci114379
发表时间：
1989
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Peltonen,J;Larjava,H;Jaakkola,S;Gralnick,H;Akiyama,SK;Yamada,SS;Yamada,KM;Uitto,J
通讯作者：
Uitto,J