Human viral pathogens are pervasive in wastewater treatment center aerosols.

Human viral pathogens are pervasive in wastewater treatment center aerosols.
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DOI:
10.1016/j.jes.2017.07.015
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发表时间:
2018-05
期刊:
Journal of environmental sciences (China)
影响因子:
--
通讯作者:
Duchaine C
Duchaine C
中科院分区:
其他
文献类型:
--
作者:
Brisebois E;Veillette M;Dion-Dupont V;Lavoie J;Corbeil J;Culley A;Duchaine C

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废水处理中心(WTC)的工作人员可能容易感染病毒引起的疾病,例如普通感冒、流感和胃肠道感染。尽管有大量文献描述了废水中发现的微生物群落,但只有少数研究描述了世贸中心气溶胶的病毒成分,尽管事实上影响世贸中心工作人员的大多数疾病都是病毒起源的,并且其中一些病毒是通过空气传播的。在这项研究中,我们评估了四个世贸中心在该环境中是否存在特别令人关注的 11 种病毒病原体,并使用宏基因组方法来表征其中一个世贸中心空气中的总病毒群落。对各个世贸中心不同地点的气溶胶中病毒的存在情况进行了评估,并对四种常用空气采样器获得的结果进行了比较。我们检测到了 11 种测试病毒中的 4 种,包括人类腺病毒 (hAdV)、轮状病毒、甲型肝炎病毒 (HAV) 和 1 型单纯疱疹病毒 (HSV1)。宏基因组检测的结果发现世贸中心气溶胶中的病毒 RNA 序列非常少,但人类 DNA 病毒的序列相对丰度要高得多。
Wastewater treatment center (WTC) workers may be vulnerable to diseases caused by viruses, such as the common cold, influenza and gastro-intestinal infections. Although there is a substantial body of literature characterizing the microbial community found in wastewater, only a few studies have characterized the viral component of WTC aerosols, despite the fact that most diseases affecting WTC workers are of viral origin and that some of these viruses are transmitted through the air. In this study, we evaluated in four WTCs the presence of 11 viral pathogens of particular concern in this milieu and used a metagenomic approach to characterize the total viral community in the air of one of those WTCs. The presence of viruses in aerosols in different locations of individual WTCs was evaluated and the results obtained with four commonly used air samplers were compared. We detected four of the eleven viruses tested, including human adenovirus (hAdV), rotavirus, hepatitis A virus (HAV) and Herpes Simplex virus type 1 (HSV1). The results of the metagenomic assay uncovered very few viral RNA sequences in WTC aerosols, however sequences from human DNA viruses were in much greater relative abundance.
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