Lymphoproliferative responses and protection in conventional piglets inoculated orally with virulent or attenuated porcine epidemic diarrhoea virus.
Lymphoproliferative responses and protection in conventional piglets inoculated orally with virulent or attenuated porcine epidemic diarrhoea virus.
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DOI：
10.1016/s0166-0934(02)00063-0
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发表时间：
2002-08
影响因子：
3.1
通讯作者：
Rubio P
中科院分区：
文献类型：
--
作者：
de Arriba ML;Carvajal A;Pozo J;Rubio P
接种猪流行性腹泻病毒（PEDV） CV-777毒株强毒株或减毒株，并在21天后用该病毒强毒株攻击常规仔猪，观察其粘膜（肠系膜淋巴结）和全身（脾脏和血液）淋巴组织的淋巴细胞增殖反应。研究人员开发了一种淋巴细胞增殖试验，在接种后和攻毒后不同时期从淋巴组织中分离出的单核细胞，用从感染pedv的细胞培养物中获得的半纯化抗原进行二次体外刺激。在接种后4 ~ 21天，猪的淋巴细胞出现了强烈的增殖反应，特别是在肠系膜淋巴结和血液中；然而，在脾脏中，这种反应较低且不太规律。经减毒后的猪对该病毒的反应较弱，淋巴细胞的增殖也与肠系膜淋巴结的单核细胞增殖相对应。淋巴细胞增殖反应显示出对同源强毒PEDV攻击的保护高度相关，并且这种相关性在肠道相关淋巴组织（肠系膜淋巴结）中更高。血液中检测到的细胞增殖反应与肠系膜淋巴结中检测到的细胞增殖反应相一致，并且也显示出良好的保护相关性。结果证实，通过淋巴细胞增殖反应评估的t细胞辅助功能有助于建立针对PEDV感染的保护性免疫反应。
Lymphocyte proliferative responses were evaluated in mucosal (mesenteric lymph nodes) and systemic (spleen and blood) lymphoid tissues of conventional piglets inoculated with the virulent or attenuated isolates of porcine epidemic diarrhoea virus (PEDV) strain CV-777 and challenged 21 days later with the virulent isolate of the same virus. A lymphoproliferative assay was developed in which mononuclear cells isolated from lymphoid tissues at different postinoculation and postchallenge days underwent a secondary in vitro stimulation with semipurified antigen obtained from PEDV-infected cell cultures. Vigorous lymphocyte proliferative responses were detected in the pigs inoculated with the virulent PEDV at postinoculation days 4–21, especially in the mesenteric lymph nodes and the blood; however, in the spleen this response was lower and less regular. The pigs inoculated with the attenuated virus showed a less intense response, the higher lymphocyte proliferation also corresponded to the mononuclear cells from mesenteric lymph nodes. Lymphocyte proliferation responses showed high correlations with protection against homologous challenge with virulent PEDV, and this correlation was higher in the gut associated lymphoid tissues (mesenteric lymph nodes). The cell proliferation response detected in blood mirrored that detected in the mesenteric lymph nodes, and showed also good correlation with protection. The results confirm that T-cell-helper function, assessed by lymphocyte proliferation responses, contributes to establishing a protective immune response against PEDV infections.
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影响因子：
2.6
作者：
Carvajal A;Lanza I;Diego R;Rubio P;Cármenes P
通讯作者：
Cármenes P
影响因子：
1.8
作者：
de Arriba, ML;Carvajal, A;Rubio, P
通讯作者：
Rubio, P
影响因子：
1.8
作者：
de Arriba ML;Carvajal A;Pozo J;Rubio P
通讯作者：
Rubio P
影响因子：
5.5
作者：
Kweon CH;Kwon BJ;Lee JG;Kwon GO;Kang YB
通讯作者：
Kang YB
DOI：
10.1016/0167-5699(96)80579-2
发表时间：
1996-02-01
期刊：
IMMUNOLOGY TODAY
影响因子：
--
作者：
Kagnoff, MF
通讯作者：
Kagnoff, MF